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Article
Erratum to: In vitro cultures of ectodermal monolayers from the model sea anemone Nematostella vectensis
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Article
In vitro cultures of ectodermal monolayers from the model sea anemone Nematostella vectensis
We report here a novel approach for the extraction, isolation and culturing of intact ectodermal tissue layers from a model marine invertebrate, the sea anemone Nematostella vectensis. A methodology is described ...
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Article
De novo emerged stemness signatures in epithelial monolayers developed from extirpated palleal buds
In botryllid ascidians, astogeny is executed through blastogenesis, a weekly, highly synchronized phenomenon of growth and death cycles, each constitutes four major developmental stages (A–D), operating simult...
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Article
Further portrayal of epithelial monolayers emergent de novo from extirpated ascidians palleal buds
Astogeny in botryllid ascidians is executed by highly synchronized, repeated development and death cycles operating simultaneously on three coexisting asexually derived generations: zooids, primary buds, and s...
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Article
Employing of the Amplified Fragment Length Polymorphism (AFLP) Methodology as an Efficient Population Genetic Tool for Symbiotic Cnidarians
Although the use of molecular markers in population genetics of marine organisms is increasingly employed, methodologic limitations still hampered the research for some taxa, such as symbiotic cnidarians, incl...
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Article
Epithelial cell cultures from Botryllus schlosseri palleal buds: accomplishments and challenges
This study focuses on recent improvement in epithelial monolayer cultures originating from whole extirpated Botryllus schlosseri (Urochordata) buds. Buds (n = 2,000) were taken at different (‘A’ to ‘D’) blastogen...
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Article
Initiation of epithelial cell cultures from palleal buds of Botryllus schlosseri, a colonial tunicate
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Article
In vitro culture of blood cells from the colonial protochordateBotryllus schlosseri
Primary cultures of circulatory blood cells from the colonial tunicateBotryllus schlosseri were cultivated in 96-well plates for up to 3 mo. in a medium based on Dulbecco’s modified Eagle’s medium, supplemented w...