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  1. No Access

    Article

    eIF5B and eIF1A reorient initiator tRNA to allow ribosomal subunit joining

    Translation initiation defines the identity and quantity of a synthesized protein. The process is dysregulated in many human diseases1,2. A key commitment step is when the ribosomal subunits join at a translation...

    Christopher P. Lapointe, Rosslyn Grosely, Masaaki Sokabe, Carlos Alvarado… in Nature (2022)

  2. No Access

    Article

    eIF5B gates the transition from translation initiation to elongation

    Translation initiation determines both the quantity and identity of the protein that is encoded in an mRNA by establishing the reading frame for protein synthesis. In eukaryotic cells, numerous translation ini...

    **fan Wang, Alex G. Johnson, Christopher P. Lapointe, Junhong Choi… in Nature (2019)

  3. No Access

    Article

    Unbiased screen of RNA tailing activities reveals a poly(UG) polymerase

    Ribonucleotidyl transferases (rNTases) add untemplated ribonucleotides to diverse RNAs. We have developed TRAID-seq, a screening strategy in Saccharomyces cerevisiae to identify sequences added to a reporter RNA ...

    Melanie A. Preston, Douglas F. Porter, Fan Chen, Natascha Buter… in Nature Methods (2019)

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    Protocol

    RNA Tagging: Preparation of High-Throughput Sequencing Libraries

    Protein–RNA networks, in which a single protein binds and controls multiple mRNAs, are central in biological control. As a result, methods to identify protein–RNA interactions that occur in vivo are valuable. ...

    Christopher P. Lapointe, Marvin Wickens in RNA Detection (2018)

  5. No Access

    Article

    Protein-RNA networks revealed through covalent RNA marks

    A fusion of RNA-binding proteins (RBPs) to a poly(U) polymerase allows the tagging of endogenous RNAs bound by the RBPs with a U-tail that can be used to identify the bound RNA by sequencing. RNA tagging is su...

    Christopher P Lapointe, Daniel Wilinski, Harriet A J Saunders… in Nature Methods (2015)

  6. Article

    Open Access

    RNA regulatory networks diversified through curvature of the PUF protein scaffold

    Proteins bind and control mRNAs, directing their localization, translation and stability. Members of the PUF family of RNA-binding proteins control multiple mRNAs in a single cell, and play key roles in develo...

    Daniel Wilinski, Chen Qiu, Christopher P. Lapointe, Markus Nevil… in Nature Communications (2015)

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