-
Protocol
Analysis of Centrosome Function and Microtubule Dynamics by Time-Lapse Microscopy in Xenopus Egg Extracts
Centrosomes are essential organelles that organize the microtubule cytoskeleton during interphase and mitosis. Centrosomes are assembled from tens to hundreds of proteins, but how these proteins are organized ...
-
Article
A Ran signalling pathway mediated by the mitotic kinase Aurora A in spindle assembly
The activated form of Ran (Ran-GTP) stimulates spindle assembly in Xenopus laevis egg extracts, presumably by releasing spindle assembly factors, such as TPX2 (target protein for Xenopus kinesin-like protein 2) a...
-
Article
Ran stimulates spindle assembly by altering microtubule dynamics and the balance of motor activities
The guanosine tri-phosphatase Ran stimulates assembly of microtubule spindles. However, it is not known what aspects of the microtubule cytoskeleton are subject to regulation by Ran in mitosis. Here we show th...
-
Article
A new function for the γ -tubulin ring complex as a microtubule minus-end cap
Microtubule nucleation from centrosomes involves a lockwasher-shaped protein complex containing γ-tubulin, named the γ-tubulin ring complex (γTuRC). Here we investigate the mechanism by which the γTuRC nucleat...