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Protocol
Data Sharing and Reuse: A Method by the AIRR Community
of (AIRR, i.e., IG and TR ) has revolutionized the ability to study the adaptive immune response via large-scale experiments. Since 2009, AIRR sequencing (AIRR-seq) has been widely applied to survey the im...
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Protocol
Adaptive Immune Receptor Repertoire (AIRR) Community Guide to TR and IG Gene Annotation
High-throughput sequencing of adaptive immune receptor repertoires (AIRR, i.e., IG and TR) has revolutionized the ability to carry out large-scale experiments to study the adaptive immune response. Since the m...
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Article
ALDH4A1 is an atherosclerosis auto-antigen targeted by protective antibodies
Cardiovascular disease (CVD) is the leading cause of mortality in the world, with most CVD-related deaths resulting from myocardial infarction or stroke. The main underlying cause of thrombosis and cardiovascu...
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Protocol
Expression Cloning of Antibodies from Single Human B Cells
The majority of lymphomas originate from B cells at the germinal center stage. Preferential selection of B-cell clones by a limited set of antigens has been suggested to drive lymphoma development. While recen...
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Article
Adaptive Immune Receptor Repertoire Community recommendations for sharing immune-repertoire sequencing data
High-throughput sequencing of B and T cell receptors is routinely being applied in studies of adaptive immunity. The Adaptive Immune Receptor Repertoire (AIRR) Community was formed in 2015 to address issues in...
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Article
Open AccesssciReptor: analysis of single-cell level immunoglobulin repertoires
The sequencing of immunoglobulin (Ig) transcripts from single B cells yields essential information about Ig heavy:light chain pairing, which is lost in conventional bulk sequencing experiments. The previously ...
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Article
The promise and challenge of high-throughput sequencing of the antibody repertoire
Georgiou and colleagues discuss rapidly evolving methods for high-throughput sequencing of the antibody repertoire, and how the resulting data may be applied to answer basic and translational research questions.
