-
Protocol
Controlling the Subcellular Localization of Signaling Proteins Using Chemically Induced Dimerization and Optogenetics
A given protein can perform numerous roles in a cell with its participation in protein complexes and distinct localization within the cell playing a critical role in its diverse functions. Thus, the ability to...
-
Protocol
Migration of Dictyostelium discoideum to the Chemoattractant Folic Acid
Dictyostelium discoideum can be grown axenically in a cultured media or in the presence of a natural food source, such as the bacterium Klebsiella aerogenes (KA). Here we describe the advantages and methods for ...
-
Article
Identification of a farnesol analog as a Ras function inhibitor using both an in vivo Ras activation sensor and a phenotypic screening approach
Mutations in Ras isoforms such as K-Ras, N-Ras, and H-Ras contribute to roughly 85, 15, and 1 % of human cancers, respectively. Proper membrane targeting of these Ras isoforms, a prerequisite for Ras activity,...
-
Chapter
The Chemotactic Compass
Cells have an amazing ability to sense very shallow gradients of chemoattractants and move directionally. This fundamental process is critical for development and numerous disease states. Dictyostelium has emerge...
-
Protocol
FRAP Analysis of Chemosensory Components of Dictyostelium
Dictyostelium discoideum is a useful cell model for studying protein–protein interactions and deciphering complex signaling pathways similar to those found in mammalian systems. Many of these inter...
