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Article
Nine 3-ketoacyl-CoA thiolases (KATs) and acetoacetyl-CoA thiolases (ACATs) encoded by five genes in Arabidopsis thaliana are targeted either to peroxisomes or cytosol but not to mitochondria
The sub-cellular location of enzymes of fatty acid β-oxidation in plants is controversial. In the current debate the role and location of particular thiolases in fatty acid degradation, fatty acid synthesis an...
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Article
Open AccessAn in silico analysis of the mitochondrial protein import apparatus of plants
An in silico analysis of the mitochondrial protein import apparatus from a variety of species; including Chlamydomonas reinhardtii, Chlorella variabilis, Ectocarpus siliculosus, Cyanidioschyzon merolae, Physcomit...
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Article
How do plants make mitochondria?
Plant mitochondria can differ in size, shape, number and protein content across different tissue types and over development. These differences are a result of signaling and regulatory processes that ensure mit...
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Article
Open AccessThe dual targeting ability of type II NAD(P)H dehydrogenases arose early in land plant evolution
Type II NAD(PH) dehydrogenases are located on the inner mitochondrial membrane of plants, fungi, protists and some primitive animals. However, recent observations have been made which identify several Arabidop...
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Protocol
In Vitro and In Vivo Protein Uptake Studies in Plant Mitochondria
The study of protein uptake into mitochondria is an important tool for investigating the subcellular distribution of proteins and the molecular mechanisms that determine location. Here we describe five techniq...
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Article
The PPR protein SLOW GROWTH 4 is involved in editing of nad4 and affects the splicing of nad2 intron 1
SLO4 is a mitochondrial PPR protein that is involved in editing nad4, possibly required for the efficient splicing of nad2 intron1.
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Article
Open AccessTo Mia or not to Mia: stepwise evolution of the mitochondrial intermembrane space disulfide relay
The disulfide relay system found in the intermembrane space (IMS) of mitochondria is an essential pathway for the import and oxidative folding of IMS proteins. Erv1, an essential member of this pathway, has be...
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Protocol
Assessment of Mitochondrial Protein Topology and Membrane Insertion
Analyzing the membrane integrity and topology of a mitochondrial protein is essential for truly understanding its function. In this chapter, we demonstrate how to analyze mitochondrial membrane proteins using ...
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Article
Open AccessRecurrent evolutionary switches of mitochondrial cytochrome c maturation systems in Archaeplastida
Mitochondrial cytochrome c maturation (CCM) requires heme attachment via distinct pathways termed systems I and III. The mosaic distribution of these systems in Archaeplastida raises questions about the genetic m...
