Isolation of Plant Organelles and Structures
Methods and Protocols
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Article
Staple crops equipped for alkaline soils
Gene editing boosts the yields of sorghum, rice and millet grown in alkaline soils.
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Metabolic evidence for distinct pyruvate pools inside plant mitochondria
The majority of the pyruvate inside plant mitochondria is either transported into the matrix from the cytosol via the mitochondria pyruvate carrier (MPC) or synthesized in the matrix by alanine aminotransferas...
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Protocol
High-Throughput Oxygen Consumption Measurements in Leaf Tissue Using Oxygen Sensitive Fluorophores
Respiratory rate measurements are crucial assays to understand mitochondrial biochemistry as well as metabolic regulation within tissues. Several technologies currently exist that can measure plant respiratory...
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Assessing the Kinetics of Metabolite Uptake and Utilization by Isolated Mitochondria Using Selective Reaction Monitoring Mass Spectrometry (SRM-MS)
Transport of tricarboxylic acid (TCA) cycle substrates across mitochondrial membranes and their subsequent oxidative decarboxylation in the matrix provide reductants for respiration-coupled ATP synthesis. Thes...
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Rapid delivery systems for future food security
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Chapter
Subcellular Proteomics as a Unified Approach of Experimental Localizations and Computed Prediction Data for Arabidopsis and Crop Plants
In eukaryotic organisms, subcellular protein location is critical in defining protein function and understanding sub-functionalization of gene families. Some proteins have defined locations, whereas others hav...
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Revolutionizing agriculture with synthetic biology
Synthetic biology is here to stay and will transform agriculture if given the chance. The huge challenges facing food, fuel and chemical production make it vital to give synthetic biology that chance—notwithst...
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Open AccessAuthor Correction: Identification of a novel cAMP dependent protein kinase A phosphorylation site on the human cardiac calcium channel
A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has been fixed in the paper.
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Open AccessTemperature-dependent metabolic adaptation of Triticum aestivum seedlings to anoxia
Wheat (Triticum aestivum) is considered anoxia intolerant but it shows variance in anoxia responses between genotypes and environmental treatments. We firstly examined 4 day old seedlings of five wheat genotypes ...
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Open AccessIdentification of a novel cAMP dependent protein kinase A phosphorylation site on the human cardiac calcium channel
The “Fight or Flight” response is elicited by extrinsic stress and is necessary in many species for survival. The response involves activation of the β-adrenergic signalling pathway. Surprisingly the mechanism...
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Open AccessThe combination of gas-phase fluorophore technology and automation to enable high-throughput analysis of plant respiration
Mitochondrial respiration in the dark (R dark) is a critical plant physiological process, and hence a reliable, efficient and high-throughput method of measuring variation in rates of R
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Open AccessInsights into the molecular basis of long-term storage and survival of sperm in the honeybee (Apis mellifera)
Honeybee males produce ejaculates consisting of large numbers of high quality sperm. Because queens never re-mate after a single mating episode early in life, sperm are stored in a specialised organ for years ...
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Open AccessMajor Cys protease activities are not essential for senescence in individually darkened Arabidopsis leaves
Papain-like Cys Proteases (PLCPs) and Vacuolar Processing Enzymes (VPEs) are amongst the most highly expressed proteases during leaf senescence in Arabidopsis. Using activity-based protein profiling (ABPP), a ...
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Book
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Protocol
The Isolation of Plant Organelles and Structures in the Post-genomic Era
In this chapter, we provide an overview of the techniques and approaches used in the isolation of plant organelles and structures. This overview shows there is a great diversity of methods currently used for t...
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Protocol
Isolation of Mitochondria, Their Sub-Organellar Compartments, and Membranes
Mitochondria are the sites of a diverse set of essential biochemical processes in plants. In order to facilitate the analysis of these functions, this chapter presents protocols for the isolation of intact mit...
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Open AccessConsequences of Nosema apis infection for male honey bees and their fertility
The queens of eusocial bees, ants and wasps mate only during a very short period early in life and males therefore produce ejaculates consisting of large numbers of high quality sperm. Such extreme selection f...
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Article
Protein turnover in plant biology
The protein content of plant cells is constantly being updated. This process is driven by the opposing actions of protein degradation, which defines the half-life of each polypeptide, and protein synthesis. Ou...
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Protocol
Activity Assay for Plant Mitochondrial Enzymes
Mitochondria are sites for respiration to produce chemical energy via oxidative phosphorylation. Their primary role has been viewed as the oxidation of organic acids via the tricarboxylic acid (TCA) cycle and ...
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Protocol
Micro-Respiratory Measurements in Plants
Respiratory measurement in plants is one of the commonly used techniques to assess metabolic activity and in vivo redox state of plant mitochondria. However, respiration rate monitoring of Arabidopsis (Arabidopsi...
