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  1. Article

    Open Access

    Cell penetration efficiency analysis of different atomic force microscopy nanoneedles into living cells

    Over the last decade, nanoneedle-based systems have demonstrated to be extremely useful in cell biology. They can be used as nanotools for drug delivery, biosensing or biomolecular recognition inside cells; or...

    Marcos Penedo, Tetsuya Shirokawa, Mohammad Shahidul Alam in Scientific Reports (2021)

  2. Article

    Open Access

    A method using electroporation for the protein delivery of Cre recombinase into cultured Arabidopsis cells with an intact cell wall

    Genome engineering in plants is highly dependent on the availability of effective molecular techniques. Despite vast quantities of research, genome engineering in plants is still limited in terms of gene deliv...

    Yuichi Furuhata, Ayako Sakai, Tomi Murakami, Mone Morikawa in Scientific Reports (2019)

  3. Article

    Open Access

    Oscillating high-aspect-ratio monolithic silicon nanoneedle array enables efficient delivery of functional bio-macromolecules into living cells

    Delivery of biomolecules with use of nanostructures has been previously reported. However, both efficient and high-throughput intracellular delivery has proved difficult to achieve. Here, we report a novel mat...

    Daisuke Matsumoto, Ramachandra Rao Sathuluri, Yoshio Kato in Scientific Reports (2015)