-
Article
Open AccessDevelopment of polyclonal antisera against movement proteins from three poleroviruses infecting cucurbits
Cucurbit aphid-borne yellows virus (CABYV), melon aphid-borne yellows virus (MABYV) and suakwa aphid-borne yellows virus (SABYV) are three poleroviruses that infect cucurbit crops. Develo** specific antisera...
-
Article
Open AccessDevelopment of polyclonal antiserum against movement protein from Potato leafroll virus and its application for the virus detection
The serological method is one of the most important techniques extensively used in crop production to detect different pathogens, especially plant viruses. An antiserum is essential for serological tests. The ...
-
Article
Open AccessBrassica yellows virus’ movement protein upregulates anthocyanin accumulation, leading to the development of purple leaf symptoms on Arabidopsis thaliana
Poleroviruses are widely distributed and often of great economic importance because they cause a variety of symptoms, such as the rolling of young leaves, leaf color changes, and plant decline, in infected pla...
-
Article
Open AccessSynergistic infection of BrYV and PEMV 2 increases the accumulations of both BrYV and BrYV-derived siRNAs in Nicotiana benthamiana
Viral synergism is caused by co-infection of two unrelated viruses, leading to more severe symptoms or increased titres of one or both viruses. Synergistic infection of phloem-restricted poleroviruses and umbr...
-
Article
Complete genome sequence analysis identifies a new genotype of brassica yellows virus that infects cabbage and radish in China
For brassica yellows virus (BrYV), proposed to be a member of a new polerovirus species, two clearly distinct genotypes (BrYV-A and BrYV-B) have been described. In this study, the complete nucleotide sequences...
-
Article
Open AccessInfection of Beet necrotic yellow vein virus with RNA4-encoded P31 specifically up-regulates pathogenesis-related protein 10 in Nicotiana benthamiana
Beet necrotic yellow vein virus (BNYVV) is the infectious agent of sugar beet rhizomania, which consists of four or five plus-sense RNAs. RNA4 of BNYVV is not essential for virus propagation in Nicotiana benthami...
-
Article
Nucleotide sequence of a chickpea chlorotic stunt virus relative that infects pea and faba bean in China
We determined the genome sequence of a new polerovirus that infects field pea and faba bean in China. Its entire nucleotide sequence (6021 nt) was most closely related (83.3% identity) to that of an Ethiopian ...
-
Article
Complete genomic sequence analysis reveals a novel fabavirus infecting cucurbits in China
The complete genome sequence of a cucurbit-infecting fabavirus was determined. Sequence analysis revealed that it had a genomic organization typical of fabaviruses, with genome segment sizes of 5870 nt (RNA-1)...
-
Article
Open AccessRapid detection of wheat yellow mosaic virus by reverse transcription loop-mediated isothermal amplification
For the detection of wheat yellow mosaic virus (WYMV), we established a reverse transcription loop-mediated isothermal amplification (RT-LAMP) method. Using Primer Explorer software, four sets of primers were ...
-
Article
Molecular characterization of two genotypes of a new polerovirus infecting brassicas in China
The genomic RNA sequences of two genotypes of a brassica-infecting polerovirus from China were determined. Sequence analysis revealed that the virus was closely related to but significantly different from turn...
-
Article
A novel strain of Beet western yellows virus infecting sugar beet with two distinct genotypes differing in the 5′-terminal half of genome
The complete genomic sequences of two distinct Beet western yellows virus (BWYV) genotypes infecting sugar beet in Bei**g, named as BWYV-BJA and BWYV-BJB (GenBank accession number HM804471, HM804472, respectivel...
-
Article
Molecular characterization of two Chinese isolates of Beet western yellows virus infecting sugar beet
Beet western yellows virus (BWYV) has previously been reported as an agent of sugar beet yellowing disease in China. In this article, the complete genomic RNA sequences of two Chinese BWYV isolates infecting beet...
-
Article
Complete sequence analysis reveals two distinct poleroviruses infecting cucurbits in China
The complete RNA genomes of a Chinese isolate of cucurbit aphid-borne yellows virus (CABYV-CHN) and a new polerovirus tentatively referred to as melon aphid-borne yellows virus (MABYV) were determined. The ent...