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    Article

    Increased chalcopyrite bioleaching capabilities of extremely thermoacidophilic Metallosphaera sedula inocula by mixotrophic propagation

    Extremely thermoacidophilic Crenarchaeota belonging to the order Sulfolobales, such as Metallosphaera sedula, are metabolically versatile and of great relevance in bioleaching. However, the impacts of extreme the...

    Chenbing Ai, Zhang Yan, Hongsheng Chai in Journal of Industrial Microbiology & Biote… (2019)

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    Article

    Identification and Analysis of a Novel Gene Cluster Involves in Fe2+ Oxidation in Acidithiobacillus ferrooxidans ATCC 23270, a Typical Biomining Acidophile

    Iron-oxidizing Acidithiobacillus spp. are applied worldwide in biomining industry to extract metals from sulfide minerals. They derive energy for survival through Fe2+ oxidation and generate Fe3+ for the dissolut...

    Chenbing Ai, Yuting Liang, Bo Miao, Miao Chen, Weimin Zeng in Current Microbiology (2018)

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    Article

    Evolution of copper arsenate resistance for enhanced enargite bioleaching using the extreme thermoacidophile Metallosphaera sedula

    Adaptive laboratory evolution (ALE) was employed to isolate arsenate and copper cross-resistant strains, from the copper-resistant M. sedula CuR1. The evolved strains, M. sedula ARS50-1 and M. sedula ARS50-2, con...

    Chenbing Ai, Samuel McCarthy, Yuting Liang in Journal of Industrial Microbiology & Biote… (2017)

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    Article

    Increased acid resistance of the archaeon, Metallosphaera sedula by adaptive laboratory evolution

    Extremely thermoacidophilic members of the Archaea such as the lithoautotroph, Metallosphaera sedula, are among the most acid resistant forms of life and are of great relevance in bioleaching. Here, adaptive labo...

    Chenbing Ai, Samuel McCarthy in Journal of Industrial Microbiology & Biote… (2016)

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    Article

    Expression, Purification, and Characterization of an Iron Chaperon Protein CyaY from Acidithiobacillus ferrooxidans

    CyaY is the bacterial homolog of frataxin, proposed to be involved in the assembly of iron–sulfur clusters. While, the physiological iron donor for the iron–sulfur clusters assembly remains controversial. In t...

    Chenbing Ai, Hongyu Mo, Qian Chen, Yuandong Liu, Lin Tang, Juan Du in Current Microbiology (2011)

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    Article

    Expression, Purification and Molecular Structure Modeling of Thioredoxin (Trx) and Thioredoxin Reductase (TrxR) from Acidithiobacillus ferrooxidans

    The thioredoxin system consists of thioredoxin (Trx), thioredoxin reductase (TrxR) and NADPH, which plays several key roles in maintaining the redox environment of the cell. In Acidithiobacillus ferrooxidans, thi...

    Yi** Wang, **aojian Zhang, Qing Liu, Chenbing Ai, Hongyu Mo in Current Microbiology (2009)