Cell Polarity Signaling
Methods and Protocols
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Protocol
Assays for Apical Constriction Using the Xenopus Model
Apical constriction refers to the active, actomyosin-driven process that reduces apical cell surface area in epithelial cells. Apical constriction is utilized in epithelial morphogenesis during embryonic devel...
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Article
Open AccessSTRAP regulates alternative splicing fidelity during lineage commitment of mouse embryonic stem cells
Alternative splicing (AS) is involved in cell fate decisions and embryonic development. However, regulation of these processes is poorly understood. Here, we have identified the serine threonine kinase recepto...
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Protocol
Animal Cap Assay for TGF-β Signaling
TGF-β signals regulate a variety of processes during early vertebrate development, from stem cell maintenance and differentiation to tissue patterning and organogenesis. Detailed understanding of how this sign...
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Article
Regulation of cell cycle progression and gene expression by H2A deubiquitination
An enzyme, Ubp-M, is identified that removes the ubiquitin modification from histone H2A. Depletion of Ubp-M leads to slower cell growth and abnormal chromosome segregation in mitosis. In addition, depletion o...
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Article
Twisted gastrulation can function as a BMP antagonist
Bone morphogenetic proteins (BMPs), including the fly homologue Decapentaplegic (DPP), are important regulators of early vertebrate and invertebrate dorsal–ventral development1,2,3,4,5,6. An evolutionarily conser...
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Chapter
Neural Induction in the Frog Xenopus laevis
During vertebrate gastrulation, the cells of the dorsal ectoderm give rise to the central nervous system; ventral ectoderm differentiates into epidermis. In amphibians, the neuralization of the dorsal ectoderm...
