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Article
Open AccessDoes phosphorylation of cofilin affect the progression of human bladder cancer?
We determined the differently expressed protein profiles and their functions in bladder cancer tissues with the aim of identifying possible target proteins and underlying molecular mechanisms for taking part i...
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Article
Antioxidant and antimicrobial activities of Smilax china L. leaf extracts
Antioxidant and antimicrobial activities of Smilax china L. leaf extracts obtained with methanol, ethanol, acetone, and water were investigated. Antioxidant activity was evaluated by determining the DPPH radical ...
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Article
Functional analysis of a BarX homologue (SngA) as a pleiotropic regulator in Streptomyces natalensis
The prior sequencing of the upstream region of the γ-butyrolactone autoregulator receptor gene (sngR) in Streptomyces natalensis revealed the presence of a 972-bp gene encoding a BarX homologue (SngA), which acts...
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Article
Open AccessAnti-tumor immunostimulatory effect of heat-killed tumor cells
AS a part of our ongoing search for a safe and efficient anti-tumor vaccine, we attempted to determine whether the molecular nature of certain tumor antigens would influence immune responses against tumor cell...
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Article
Identification of the bkdAB gene cluster, a plausible source of the starter-unit for virginiamycin M production in Streptomyces virginiae
The bkdAB gene cluster, which encodes plausible E1 and E2 components of the branched-chain α-keto acid dehydrogenase (BCDH) complex, was isolated from Streptomyces virginiae in the vicinity of a regulatory island...
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Article
Spleen tyrosine kinase participates in src-mediated migration and proliferation by PDGF-BB in rat aortic smooth muscle cells
Tyrosine kinases, Src and spleen tyrosine kinase (Syk), play crucial roles in cell responses to platelet-derived growth factor (PDGF) and may have their functional interactions. In this study, we focused on in...
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Article
Phorbol ester-induced contraction through p38 mitogen-activated protein kinase is diminished in aortas from DOCA-salt hypertensive rats
The role of mitogen-activated protein kinase (MAPK) in the decreased contractile response to phorbol ester in aortic smooth muscle strips from deoxycorticosterone acetate (DOCA)-salt hypertensive rats was exam...
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Article
Cloning and in vivo functional analysis by disruption of a gene encoding the γ-butyrolactone autoregulator receptor from Streptomyces natalensis
A gene encoding a γ-butyrolactone autoregulator receptor, which has a common activity as DNA-binding transcriptional repressors controlling secondary metabolism and/or morphological differentiation in Streptomyce...
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Article
Cloning and characterization of a gene encoding the γ-butyrolactone autoregulator receptor from Streptomyces clavuligerus
With primers designed for the conserved region of the γ-butyrolactone autoregulator receptor proteins from Streptomyces species, PCR using the Streptomyces clavuligerus genome DNA as a template gave a clear band ...
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Article
Intergeneric conjugal transfer of plasmid DNA from Escherichia coli to Kitasatospora setae, a bafilomycin B1 producer
An effective transformation procedure for Kitasatospora setae was established based on transconjugation from Escherichia coli ET12567 (pUZ8002) using a ϕC31-derived integration vector, pSET152, containing oriT an...
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Article
Identification by gene deletion analysis of barB as a negative regulator controlling an early process of virginiamycin biosynthesis in Streptomyces virginiae
The Streptomyces virginiae γ-butyrolactone autoregulator virginiae butanolide is a low-molecular-weight Streptomyces hormone eliciting virginiamycin biosynthesis through its binding to the specific receptor prote...
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Article
γ-Butyrolactone autoregulators and receptor proteins in non-Streptomyces actinomycetes producing commercially important secondary metabolites
The presence of γ-butyrolactone autoregulators and their receptor proteins were investigated in five representative strains of non-Streptomyces actinomycetes producing commercially important secondary metabolite...
