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Article
Open AccessMale meiotic spindle poles are stabilized by TACC3 and cKAP5/chTOG differently from female meiotic or somatic mitotic spindles in mice
Transforming acidic acid coiled-coil protein 3 (TACC3) and cytoskeleton associated protein 5 (cKAP5; or colonic hepatic tumor overexpressed gene, chTOG) are vital for spindle assembly and stabilization initiat...
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Article
Open AccessSeparation and Loss of Centrioles From Primordidal Germ Cells To Mature Oocytes In The Mouse
Oocytes, including from mammals, lack centrioles, but neither the mechanism by which mature eggs lose their centrioles nor the exact stage at which centrioles are destroyed during oogenesis is known. To answer...
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Article
Open AccessAuthor Correction: A novel atypical sperm centriole is functional during human fertilization
In the original version of this Article, the affiliation details for Jadranka Loncarek and Vito Mennella were incorrectly given as ‘Cell Biology Program, The Hospital for Sick Children, Department of Biochemis...
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Article
Open AccessA novel atypical sperm centriole is functional during human fertilization
The inheritance of the centrosome during human fertilization remains mysterious. Here we show that the sperm centrosome contains, in addition to the known typical barrel-shaped centriole (the proximal centriol...
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Protocol
Utility of Animal Models for Human Embryo Culture: Nonhuman Primates
Nonhuman primates are the closest relatives to humans and therefore our most evolutionary close cousins. While marvelous insights are gleaned from studying rodents and other systems, it is impossible to envisi...
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Article
Systems biology discoveries using non-human primate pluripotent stem and germ cells: novel gene and genomic imprinting interactions as well as unique expression patterns
The study of pluripotent stem cells has generated much interest in both biology and medicine. Understanding the fundamentals of biological decisions, including what permits a cell to maintain pluripotency, tha...
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Article
Ubiquitin tag for sperm mitochondria
Like other mammals, humans inherit mitochondria from the mother only, even though the sperm contributes nearly one hundred mitochondria to the fertilized egg. In support of the idea that this strictly maternal...
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Article
The paternal inheritance of the centrosome, the cell's microtubule-organizing center, in humans, and the implications for infertility
Successful fertilization in humans, achieved when parental chromosomes intermix at first mitosis, requires centrosome restoration and microtubule-mediated motility. Imaging of inseminated human oocytes reveals...
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Article
Kinetochore appearance during meiosis, fertilization and mitosis in mouse oocytes and zygotes
The events of mammalian fertilization overlap with the completion of meiosis and first mitosis; the pronuclei never fuse, instead the parental genomes first intermix at the mitotic spindle equator at metaphase...
