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Article
AChE uses
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Protocol
The Isolation of High Molecular Weight Eukaryotic DNA
The isolation of high molecular weight eukaryotic DNA in good yield is an important prerequisite for the analysis of specific sequences by Southern blotting (Chapter 9
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Protocol
Radiolabeling of DNA by Nick Translation
Nick translation is the name given to a reaction that is used to replace cold nucleoside triphosphates in a double-stranded DNA molecule with radioactive ones (1,2). Free 3′-hydroxyl groups are created within the...
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Protocol
Detection of Specific DNA Sequences—The Southern Transfer
The purpose of this technique is the detection and characterization of specific DNA sequences. The DNA is fragmented by digestion with a restriction endonuclease, and the fragments separated by agarose gel ele...
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Article
A linked genetic marker for multiple endocrine neoplasia type 2A on chromosome 10
Multiple endocrine neoplasia type 2A (MEN2A) is an autosomal dominantly inherited cancer syndrome characterized by medullary carcinoma of the thyroid, phaeochromocytoma and hyperparathyroidism. Almost all gen...
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Article
Deletion of genes on chromosome 1 in endocrine neoplasia
Recent studies have identified normal cellular DNA sequences which are lost in the development of embryonal1–6 and adult7,8 tumours. These tumours are thought to arise after a primary mutation in one allele of s...
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Article
Early diagnosis of multiple endocrine neoplasia type IIa
We report on incidental findings during family screening of two kindreds with multiple endocrine neoplasia type IIa. Pheochromocytoma and medullary thyroid carcinoma of considerable size were detected. The res...
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Article
Paternal origin of new mutations in Von Recklinghausen neurofibromatosis
VON Recklinghausen neurofibromatosis (NF-1)1–4 is a common autosomal dominant disorder. The estimated new mutation rate (1 x 10−4) is one of the highest for a human disorder1. Here we report that in 12 of 14 fam...
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Article
Abstracts of poster presentations