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Chapter
Present Status of Research on Hematoporphyrin Derivatives and Their Photophysical Properties
Hematoporphyrin (Hp)-induced photosensitivity was first reported in mice by Hausmann (1911), and in man, following a self-experiment, by Meyer-Betz (1913). In the late 1950s and early 1960s, the ability of Hp ...
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Article
Endocapsular lavage with photofrin II as a photodynamic therapy for lens epithelial proliferation
The most common and visually significant complication of both extracapsular and endocapsular cataract extractions is the formation of a secondary cataract because of the proliferation of retained lens epitheli...
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Chapter and Conference Paper
Interaction of Q-Switched and Mode-Locked Nd: YAG Laser Pulsed with Ocular Media: An Experimental Analysis
We report on a wide series of experiments performed with the aim of clarifying basic aspects of the interaction between short and ultrashort laser pulses and ocular media which occur in the photodisruption of ...
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Chapter
Laser Applications in Bio-Medicine: Tumor Therapy and Localization Using Photosensitizing Drugs
Among the various applications of lasers in Biomedicine, a particularly interesting one makes use of a laser beam and photosensitizing drugs, for therapeutic and diagnostic purposes. Out of the several possibi...
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Article
A 580 nm emission in haematoporphyrin-derivative solution and in treated cells
Continuous wave and time-resolved fluorescence microscopy have been extensively used to characterize the behaviour of haematoporphyrin derivative (HPD) both in solution and in single cells. In this work, we re...
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Chapter
Time-Resolved Fluorescence Microscopy: Examples of Applications to Biology
Fluorescence microscopy (microfluorimetry) is an established technique that provides useful information about the biomolecules in a cellular environment[1,2]. The technique consists in exciting the samples (ce...
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Article
In situ evaluation of the functional state of chromatin by means of Quinacrine Mustard staining and time-resolved fluorescence microscopy
A pulsed laser microfluorometer with high spatial and temporal resolution was employed to study the functional state of chromatin, using Quinacrine Mustard as the fluorescent probe. Corresponding segments of p...
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Article
Laser-biophysics
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Chapter
Laser Fluorescent Microirradiation: A New Technique
The technique of fluorescence microscopy has been increasingly used for structural studies of biologic molecules (Konev, 1967; Udenfried, 1969; Duchesne, 1973). This technique is particularly valuable and sens...
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Book
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Chapter and Conference Paper
Evaluation of the Chromatin Functional State by Means of Fluorescence Decay Time Analysis
This paper discusses the application of laser microfluorometric techniques, together with the use of fluorescent probes, to investigate some functional aspects of biological macromolecules at the cellular level.
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Article
Libri ricevuti e recensioni
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Article
A simple and reliable short-pulse dye laser
A simple technique is described that allows the generation of a short pulses (1 ns) from dye lasers pumped by a much longer (7 ns) N2-laser pulse. Dye-laser pulses, either with a narrow bandwidth or a short durat...
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Article
Recensioni
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Chapter and Conference Paper
Laser-Induced Fluorescence of Biological Molecules
A study is made of the laser-induced fluorescence properties of the complex formed by Quinacrine Mustard bound to Deoxyribonucleic Acid (DNA). Specific information is obtained on the binding mechanisms and on ...
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Article
Subnanosecond pulses from a single-cavity dye laser
A simple nitrogen pumped dye laser is described which, sequentially in time, generates two pulses, one due to superradiance and the other to laser action. The duration of both the pulses can be changed continu...
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Article
Pulsed tunable lasers in cytofluorometry
A tunable dye laser which generates pulses of high intensity (107 W cm−2) and short duration (2 nsec) was used in an experimental set-up for cytofluorometry. The fluorescence signal given by a single cell (frog e...
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Article
Nonlinear optics with picosecond laser pulses
Picosecond laser pulses have proved to be an important tool in the measuring of short characteristic times and in several nonlinear optical experiments. In particular they allow the observation of several effe...
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Article
Self-locking of modes in a passiveQ-switched laser
A theory is presented in this paper for self-locking of modes in a passiveQ-switched laser. Under several assumptions, which are well fulfilled in practice, it is shown that the phases of the oscillating modes ar...
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Article
On the decay time of regular spiking in a ruby laser