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Article
Open AccessRegulation of Immunoproteasome Function in the Lung
Impaired immune function contributes to the development of chronic obstructive pulmonary disease (COPD). Disease progression is further exacerbated by pathogen infections due to impaired immune responses. Elim...
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Article
Molecular alterations in proteasomes of rat liver during aging result in altered proteolytic activities
Aging induces alterations of tissue protein homoeostasis. To investigate one of the major systems catalysing intracellular protein degradation we have purified 20S proteasomes from rat liver of young (2 months...
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Article
Circulating Extracellular Proteasome in the Cerebrospinal Fluid: A Study on Concentration and Proteolytic Activity
Alterations of the intracellular ubiquitin–proteasome pathway are found in neurodegenerative and inflammatory disorders of the central nervous system, as well as in its malignancies. Inhibitory substrates of t...
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Article
Quantitative proteome analysis of the 20S proteasome of apoptotic Jurkat T cells
Regulated proteolysis plays important roles in cell biology and pathological conditions. A crosstalk exists between apoptosis and the ubiquitin–proteasome system, two pathways responsible for regulated proteol...
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Article
Reciprocal Effects of α-Synuclein Overexpression and Proteasome Inhibition in Neuronal Cells and Tissue
Defects in the 20S/26S proteasome and conformational changes in α-synuclein (α-syn) are implicated in the development of sporadic and familial cases of PD. The objective of this study was to evaluate whether α...
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Article
Polyubiquitin substrates allosterically activate their own degradation by the 26S proteasome
Activation of the 20S proteasome requires the binding of regulatory proteins such as the 19S regulatory particle, which opens the 20S gates allowing substrate access to the active sites. New data now indicate ...
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Article
Open AccessRole of proteasomes in disease
A functional ubiquitin proteasome system is essential for all eukaryotic cells and therefore any alteration to its components has potential pathological consequences. Though the exact underlying mechanism is u...
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Article
The base of the proteasome regulatory particle exhibits chaperone-like activity
Protein substrates of the proteasome must apparently be unfolded and translocated through a narrow channel to gain access to the proteolytic active sites of the enzyme. Protein folding in vivo is mediated by mole...
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Article
Alterations of proteasome activities in skeletal muscle tissue of diabetic rats
During the last years many investigations have shown that a major catalyst within the mechanism of skeletal muscle wasting occuring under conditions like sepsis, injuries, trauma, cancer cachexia, chronic acid...
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Article
Structural and functional properties of proteasome activator PA28
The proteasome activator PA28 or 11S regulator is a protein complex composed of two different but homologous polypeptides, termed PA28α and PA28β. The purified activator protein (_200 kDa) is a ring-shaped het...
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Article
The 20S/26S proteasomal pathway of protein degradation in muscle tissue
Similar to all other eukaryotic cells and tissues muscle tissue contains the proteolytic system of 20S/26S proteasomes with the 20S proteasome existing predominantly in a latent state. Unlike with the mammalia...
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Chapter and Conference Paper
Non - Lysosomal, High - Molecular - Mass Cysteine Proteinases from Rat Skeletal Muscle
It is well established that in protein metabolism protein synthesis as well as protein degradation are equally important for maintaining cellular viability. Thus, a basal level of intracellular proteolysis is ...
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Article
Drosophila small cytoplasmic 19S ribonucleoprotein is homologous to the rat multicatalytic proteinase
All eukaryotic cells so far analysed contain 19S particles which share a cylinder-like shape and are composed of a set of proteins of relative molecular mass ranging typically from 19,000 to 36,000 (refs 1–10)...
