Therapeutic Proteins
Methods and Protocols
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Protocol
An Approach for Antigen-Agnostic Identification of Virus-Like Particle-Displayed Epitopes that Engage Specific Antibody V Gene Regions
Antibody complementarity determining regions (CDRs) participate in antigen recognition, but not all participate equally in antigen binding. Here we describe a technique for discovering strong, specific binding...
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Open AccessA virus-like particle-based bivalent PCSK9 vaccine lowers LDL-cholesterol levels in non-human primates
Elevated low-density lipoprotein cholesterol (LDL-C) is an important risk factor in the development of atherosclerotic cardiovascular disease (ASCVD). Inhibitors of proprotein convertase subtilisin/kexin type ...
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Open AccessA vaccine targeting the L9 epitope of the malaria circumsporozoite protein confers protection from blood-stage infection in a mouse challenge model
Pre-erythrocytic malaria vaccines that induce high-titer, durable antibody responses can potentially provide protection from infection. Here, we engineered a virus-like particle (VLP)-based vaccine targeting a...
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Open AccessAn epitope-based malaria vaccine targeting the junctional region of circumsporozoite protein
A malaria vaccine that elicits long-lasting protection and is suitable for use in endemic areas remains urgently needed. Here, we assessed the immunogenicity and prophylactic efficacy of a vaccine targeting a ...
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Assessing Antibody Specificity in Human Serum Using Deep Sequence-Coupled Biopanning
Affinity selection using phage-display technologies is a powerful tool for identifying the peptide epitopes of monoclonal antibodies. Coupling affinity selection with deep sequencing technologies allows for th...
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Open AccessQß Virus-like particle-based vaccine induces robust immunity and protects against tauopathy
Tauopathies, including frontotemporal dementia (FTD) and Alzheimer’s disease (AD) are progressive neurodegenerative diseases clinically characterized by cognitive decline and could be caused by the aggregation...
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Open AccessA proof-of-concept study for the design of a VLP-based combinatorial HPV and placental malaria vaccine
In Africa, cervical cancer and placental malaria (PM) are a major public health concern. There is currently no available PM vaccine and the marketed Human Papillomavirus (HPV) vaccines are prohibitively expens...
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Open AccessVLP-based vaccine induces immune control of Staphylococcus aureus virulence regulation
Staphylococcus aureus is the leading cause of skin and soft tissue infections (SSTIs) and mounting antibiotic resistance requires innovative treatment strategies. S. aureus uses secreted ...
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Open AccessA malaria vaccine candidate based on an epitope of the Plasmodium falciparum RH5 protein
The Plasmodium falciparum protein RH5 is an adhesin molecule essential for parasite invasion of erythrocytes. Recent studies show that anti-PfRH5 sera have potent invasion-inhibiting activities, supporting the id...
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Erratum: The targeted delivery of multicomponent cargos to cancer cells by nanoporous particle-supported lipid bilayers
Nature Materials 10, 389–397 (2011); published online 17 April 2011; corrected online 10 May 2011. In the version of this Letter originally published, in Fig. 1 a double bond in the maleimide cycle of the cros...
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The targeted delivery of multicomponent cargos to cancer cells by nanoporous particle-supported lipid bilayers
Encapsulation of drugs within nanocarriers that selectively target malignant cells promises to mitigate side effects of conventional chemotherapy and to enable delivery of the unique drug combinations needed f...
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Virus-like Peptide Vaccines Against Aß N-terminal or C-Terminal Domains Reduce Amyloid Deposition in APP Transgenic Mice without Addition of Adjuvant
Immunotherapy against the Aß peptide is increasingly viewed as an effective means of preventing and even decreasing Aß deposition in transgenic mouse models and human cases of Alzheimer's disease. A prior acti...
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Open AccessOvercoming antigen masking of anti-amyloidbeta antibodies reveals breaking of B cell tolerance by virus-like particles in amyloidbeta immunized amyloid precursor protein transgenic mice
In prior work we detected reduced anti-Aβ antibody titers in Aβ-vaccinated transgenic mice expressing the human amyloid precursor protein (APP) compared to nontransgenic littermates. We investigated this obser...
