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  1. No Access

    Article

    Secretion of truncated recombinant rabies virus glycoprotein with preserved antigenic properties using a co-expression system in Hansenula polymorpha

    Rabies virus infection remains a serious public health threat in the develo** world, where cost-concerns make wide-scale public health interventions impractical. The development of novel and inexpensive ELIS...

    Weidong Qian, Frank Aguilar, Ting Wang, Bingsheng Qiu in Journal of Microbiology (2013)

  2. No Access

    Article

    A Novel Mucosal Vaccine Against Foot-and-Mouth Disease Virus Induces Protection in Mice and Swine

    Epitopes of a foot-and-mouth disease virus (FMDV) capsid protein VP1 complex and a chimera of 6×His-tagged cholera toxin B subunit (hCTB) were expressed in Hansenula polymorpha and used together as a mucosal vacc...

    Houhui Song, Zhiliang Wang, Dongxia Zheng, Weihuan Fang, Yong Li… in Biotechnology Letters (2005)

  3. No Access

    Article

    Design of Vectors for Efficient Integration and Transformation in Hansenula polymorpha

    Four vectors were constructed for high-level expression of heterologous proteins with high copy number and mitotic stability in Hansenula polymorpha. All of them contained the conserved H. polymorpha-derived ribo...

    Yueyong Liu, Yong Li, Liyue Liu, Xuebo Hu, Bingsheng Qiu in Biotechnology Letters (2005)

  4. No Access

    Article

    Detection of foot-and-mouth virus antibodies using a purified protein from the high-level expression of codon-optimized, foot-and-mouth disease virus complex epitopes in Escherichia coli

    A codon optimized DNA sequence coding for foot-and-mouth disease virus (FMDV) capsid protein complex epitopes of VP1 amino acid residues 21–40, 135–160, and 200–213 was genetically fused to the C-terminal end of ...

    Houhui Song, Weihuan Fang, Zhiliang Wang, Dongxia Zheng, Jian Du… in Biotechnology Letters (2004)

  5. No Access

    Article

    Expression of a human lactoferrin N-lobe in Nicotiana benthmiana with potato virus X-based agroinfection

    A DNA fragment encoding the N-terminal half (N-lobe) of the human lactoferrin (hLfN) gene has now been cloned into recombinant Potexvirus potato virus X (PVX) vector and expressed in Nicotiana benthmiana using ag...

    Yong Li, Yunfeng Geng, Houhui Song, Guangyu Zheng, Liandong Huan… in Biotechnology Letters (2004)

  6. No Access

    Article

    Development of a set of expression vectors in Hansenula polymorpha

    Four expression vectors based on formate dehydrogenase promoter (FMDp) and methanol oxidase promoter (MOXp) from Hansenula polymorpha were developed to express heterologous genes in Hansenula polymorpha. A secret...

    Houhui Song, Yong Li, Weihuan Fang, Yunfeng Geng, Xu Wang… in Biotechnology Letters (2003)

  7. No Access

    Article

    Effects of mutated replicase and movement protein genes on attenuation of tobacco mosaic virus

    Our previous reports showed that one opal mutation (UGA) and one ochre mutation (UAA) respectively located in the replicase and movement protein (MP) genes of the attenuated tomato mosaic virus K(ToMV-K) contr...

    Gong Yang, Bingsheng Qiu, Junya Wei… in Science in China Series C: Life Sciences (2001)

  8. No Access

    Article

    Recombinant constructions and infectivity analysis of tobacco mosaic virus and attenuated tomato mosaic virus N14 genomes

    The recombinant clones of pTN and pNT have been constructed by exchanging the coding regions of the movement proteins (MP), coat proteins (CP) and 3− noncoding regions between the cDNAs of the tobacco mosaic v...

    Gong Yang, **angguo Liu, Bingsheng Qiu in Chinese Science Bulletin (2000)

  9. No Access

    Article

    Design of polarizing interferometer in sub-millimeter wavelength range

    Based upon the quasi-optical technique consideration, the dependence of polarizing interferometer properties on Gaussion beam parameters were analyzed. The formulas for PI construction design and the errors of...

    Ruman Qiu, Bingsheng Qiu in International Journal of Infrared and Millimeter Waves (1996)