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Chapter
Retreat Growth in the Ascidian Botryllus Schlosseri: A Consequence of Nonself Recognition
In the genus Botryllus fusion between genetically distinct individuals is controlled by a single genetic locus (or haplotype) with multiple codominantly expressed alleles. Colonies which do not share an allele at...
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Chapter
Speculations on the Relationships of Two Botryllus Allo-Recognition Reactions—Colony Specificity and Resorption—To Vertebrate Histocompatibility
The objective of this essay is to consider functions of the vertebrate major histocompatibility complex (MHC), especially in comparison to allorecognition in colonial tunicates. Elsewhere Burnet (1971), and ou...
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Article
In vitro culture of blood cells from the colonial protochordateBotryllus schlosseri
Primary cultures of circulatory blood cells from the colonial tunicateBotryllus schlosseri were cultivated in 96-well plates for up to 3 mo. in a medium based on Dulbecco’s modified Eagle’s medium, supplemented w...
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Article
Initiation of epithelial cell cultures from palleal buds of Botryllus schlosseri, a colonial tunicate
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Chapter
Molecular Characterization of Metallothionein- and Cytochrome P4501A-CDNAS of Sparus aurata and Their Use for Detecting Pollution along the Mediterranean Coast of Israel
Members of the fish family Sparidae are abundant in various continental shelf habitats along the Mediterranean, including relatively clean and polluted regions. Fish Metallothionein (MT) and cytochrome P4501A ...
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Article
Further steps in the initiation of cell cultures from embryos and adult sponge colonies
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Article
Ex situ Culture of Colonial Marine Ornamental Invertebrates: Concepts for Domestication
The worldwide market for ornamental saltwater invertebrates supplies the needs of millions of aquarium hobbyists, as well as for public exhibition (zoos, aquaria), universities, and research institutions. The ...
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Chapter
Allorecognition and Microsatellite Allele Polymorphism of Botryllus schlosseri from the Adriatic Sea
Botryllus schlossen, a compound ascidian is found worldwide in temperate and subtropical shallow waters. This species, which originated in the Mediterranean Sea, has become cosmopolitan via the tra...
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Article
A Simple, Reliable, and Fast Protocol for Thraustochytrid DNA Extraction
DNA extraction of thraustochytrids, common marine unicellular organisms, is usually accomplished by either the cetyltrimethylammonium bromide (CTAB) or proteinase K protocols. A novel lysis buffer protocol fo...
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Article
Nubbing of Coral Colonies: A Novel Approach for the Development of Inland Broodstocks
The establishment of large numbers of ramets from any individual coral genet can be used as a valuable source of material for the trade in ornamental marine organisms and in physiological and ecotoxicological ...
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Article
Epithelial cell cultures from Botryllus schlosseri palleal buds: accomplishments and challenges
This study focuses on recent improvement in epithelial monolayer cultures originating from whole extirpated Botryllus schlosseri (Urochordata) buds. Buds (n = 2,000) were taken at different (‘A’ to ‘D’) blastogen...
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Article
Isolation by distance in the scleractinian coral Seriatopora hystrix from the Red Sea
Pelagic dispersal of larvae in sessile marine invertebrates could in principle lead to a homogeneous gene pool over vast distances, yet there is increasing evidence of surprisingly high levels of genetic diffe...
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Article
Development of Panel of Monoclonal Antibodies Specific to Urochordate Cell Surface Antigens
Monoclonal antibodies are an important tool in the study of botryllid ascidians’ immunology and developmental biology. Here we describe the development of a panel of 38 monoclonal antibodies that are specific to
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Article
Marine Invertebrate Cell Cultures: New Millennium Trends
This review analyzes activities in the field of marine invertebrate cell culture during the years 1999 to 2004 and compares the outcomes with those of the preceding decade (1988 to 1998). During the last 5 yea...
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Article
Steps in the construction of underwater coral nursery, an essential component in reef restoration acts
Many coral reefs worldwide are rapidly declining, but efficient restoration techniques are not yet available. Here, we evaluate methodologies for reef restoration based on the “gardening concept”. A floating m...
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Article
In vivo light-microscopic documentation for primary calcification processes in the hermatypic coral Stylophora pistillata
Skeletogenesis in the hermatypic coral Stylophora pistillata was studied by using the lateral skeleton preparative (LSP) assay, viz., a coral nubbin attached to a glass coverslip glued to the bottom of a Petri di...
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Article
Postglacial-period and Recent Invasions Shape the Population Genetics of Botryllid Ascidians along European Atlantic Coasts
The colonial urochordate Botryllus schlosseri is a sedentary species of Mediterranean origin that became cosmopolitan, probably because of postglacial-period dispersal and human-mediated invasions of colonies att...
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Article
Pattern of settlement and natural chimerism in the colonial urochordate Botryllus schlosseri
Colonies of the cosmopolitan urochordate Botryllus schlosseri that share one or both alleles at a single allorecognition locus (Fu/HC) and come into tissue contacts, may fuse and form a mixed entity, a chimera. B...
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Article
Open AccessCoral kin aggregations exhibit mixed allogeneic reactions and enhanced fitness during early ontogeny
Aggregated settlement of kin larvae in sessile marine invertebrates may result in a complex array of compatible and incompatible allogeneic responses within each assemblage. Each such aggregate can, therefore,...
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Article
Employing of the Amplified Fragment Length Polymorphism (AFLP) Methodology as an Efficient Population Genetic Tool for Symbiotic Cnidarians
Although the use of molecular markers in population genetics of marine organisms is increasingly employed, methodologic limitations still hampered the research for some taxa, such as symbiotic cnidarians, incl...
