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Open AccessMonitoring of species’ genetic diversity in Europe varies greatly and overlooks potential climate change impacts
Genetic monitoring of populations currently attracts interest in the context of the Convention on Biological Diversity but needs long-term planning and investments. However, genetic diversity has been largely ...
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Article
Cell Cultures from Marine Invertebrates: New Insights for Capturing Endless Stemness
Despite several decades of extensive research efforts, there is yet no single permanent cell line available from marine invertebrates as these cells stop dividing in vitro within 24–72 h after their isolation,...
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Article
Employing of the Amplified Fragment Length Polymorphism (AFLP) Methodology as an Efficient Population Genetic Tool for Symbiotic Cnidarians
Although the use of molecular markers in population genetics of marine organisms is increasingly employed, methodologic limitations still hampered the research for some taxa, such as symbiotic cnidarians, incl...
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Article
Postglacial-period and Recent Invasions Shape the Population Genetics of Botryllid Ascidians along European Atlantic Coasts
The colonial urochordate Botryllus schlosseri is a sedentary species of Mediterranean origin that became cosmopolitan, probably because of postglacial-period dispersal and human-mediated invasions of colonies att...
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Article
Steps in the construction of underwater coral nursery, an essential component in reef restoration acts
Many coral reefs worldwide are rapidly declining, but efficient restoration techniques are not yet available. Here, we evaluate methodologies for reef restoration based on the “gardening concept”. A floating m...
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Article
Development of Panel of Monoclonal Antibodies Specific to Urochordate Cell Surface Antigens
Monoclonal antibodies are an important tool in the study of botryllid ascidians’ immunology and developmental biology. Here we describe the development of a panel of 38 monoclonal antibodies that are specific to
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Article
Marine Invertebrate Cell Cultures: New Millennium Trends
This review analyzes activities in the field of marine invertebrate cell culture during the years 1999 to 2004 and compares the outcomes with those of the preceding decade (1988 to 1998). During the last 5 yea...
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Article
Isolation by distance in the scleractinian coral Seriatopora hystrix from the Red Sea
Pelagic dispersal of larvae in sessile marine invertebrates could in principle lead to a homogeneous gene pool over vast distances, yet there is increasing evidence of surprisingly high levels of genetic diffe...
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Article
A Simple, Reliable, and Fast Protocol for Thraustochytrid DNA Extraction
DNA extraction of thraustochytrids, common marine unicellular organisms, is usually accomplished by either the cetyltrimethylammonium bromide (CTAB) or proteinase K protocols. A novel lysis buffer protocol fo...
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Allorecognition and Microsatellite Allele Polymorphism of Botryllus schlosseri from the Adriatic Sea
Botryllus schlossen, a compound ascidian is found worldwide in temperate and subtropical shallow waters. This species, which originated in the Mediterranean Sea, has become cosmopolitan via the tra...