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  1. No Access

    Article

    Gene-knockout by iSTOP enables rapid reproductive disease modeling and phenoty** in germ cells of the founder generation

    Cytosine base editing achieves C•G-to-T•A substitutions and can convert four codons (CAA/CAG/CGA/TGG) into STOP-codons (induction of STOP-codons, iSTOP) to knock out genes with reduced mosaicism. iSTOP enables...

    Yaling Wang, **gwen Chen, Xueying Huang, Bangguo Wu… in Science China Life Sciences (2024)

  2. Article

    Open Access

    Dnali1 is required for sperm motility and male fertility in mice

    The sperm flagellum is an evolutionarily conserved specialized organelle responsible for sperm motility and male fertility. Deleterious mutations in genes involved in the sperm flagellum assembly can often cau...

    Yiling Zhou, Yaling Wang, **gwen Chen, Bangguo Wu… in Basic and Clinical Andrology (2023)

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  3. No Access

    Article

    Protein Production Through Microbial Conversion of Rice Straw by Multi-Strain Fermentation

    Multi-strain mixed fermentation can provide a relatively complete lignocellulosic enzyme system compared with single-strain fermentation. This study was firstly to screen strains which have a strong ability to...

    **ru Jia, Huayou Chen, Bangguo Wu, Fengjie Cui… in Applied Biochemistry and Biotechnology (2019)

  4. Article

    Impact of orientation and flexibility of peptide linkers on T. maritima lipase Tm1350 displayed on Bacillus subtilis spores surface using CotB as fusion partner

    Fusion protein construction often requires peptide linkers for prolonged conformation, extended stability and enzyme activity. In this study a series of fusion between Thermotoga maritima lipase Tm1350 and Bacill...

    Jawad Ullah, Huayou Chen, Ake Vastermark… in World Journal of Microbiology and Biotechn… (2017)

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  5. No Access

    Article

    Cations Optimization for Protein Enrichment in Rice Straw by Mixed Cultures of Neurospora crassa 14-8 and Candida utilis Using Response Surface Methodology

    This study investigated the effect of cation optimization by mixed cultures of Neurospora crassa and Candida utilis on the true protein (TP) content. Firstly, to enhance the nutritional contents of rice straw (RS...

    **ru Jia, Huayou Chen, Bangguo Wu, Zhong Ni in Applied Biochemistry and Biotechnology (2017)

  6. No Access

    Article

    Effect of Linker Length and Flexibility on the Clostridium thermocellum Esterase Displayed on Bacillus subtilis Spores

    In fusion protein design strategies, the flexibility and length of linkers are important parameters affecting the bioactivity of multifunctional proteins. A series of fusion proteins with different linkers wer...

    Huayou Chen, Bangguo Wu, Tianxi Zhang, **ru Jia… in Applied Biochemistry and Biotechnology (2017)