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Open AccessNovel Osteogenic Ti-6Al-4V Device For Restoration Of Dental Function In Patients With Large Bone Deficiencies: Design, Development And Implementation
Custom devices supporting bone regeneration and implant placement are needed for edentulous patients with large mandibular deficiencies where endosteal implantation is not possible. We developed a novel subper...
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Article
Inorganic Phosphate Induces Mammalian Growth Plate Chondrocyte Apoptosis in a Mitochondrial Pathway Involving Nitric Oxide and JNK MAP Kinase
Chondrocytes in the hypertrophic zone of the growth plate undergo apoptosis during endochondral bone development via mechanisms that involve inorganic phosphate (Pi) and nitric oxide (NO). Recent evidence sugg...
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Article
Osteoblast-Mediated Mineral Deposition in Culture is Dependent on Surface Microtopography
Osteoblast phenotypic expression in monolayer culture depends on surface microtopography. Here we tested the hypothesis that mineralized bone nodule formation in response to osteotropic agents such as bone mor...
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Article
The First Stage of Transforming Growth Factor β1 Activation is Release of the Large Latent Complex from the Extracellular Matrix of Growth Plate Chondrocytes by Matrix Vesicle Stromelysin-1 (MMP-3)
Transforming growth factor beta-1 (TGF-β1) is secreted in a biologically inactive form and stored in the extracellular matrix as a 290 kDa complex consisting of the mature TGF-βl homodimer (Mr 25 kDa), the lat...
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Article
1a,25-(OH)2D3 Regulates 25-Hydroxyvitamin D3 24R-Hydroxylase Activity in Growth Zone Costochondral Growth Plate Chondrocytes via Protein Kinase C
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Article
Tissue response and osteoinduction of human bone grafts in vivo
Freeze-dried human bone allograft is used clinically as an adjunct to autologous bone graft. When freeze-dried human bone allograft is demineralized, the allograft is osteoinductive, since it causes bone to f...
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Article
Der Differenzierungsgrad von Chondrozyten und ihre Vorbehandlung mit „platelet-derived-growth-factor“
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Article
TGFβ1 Regulates 25-Hydroxyvitamin D3 1α- and 24-Hydroxylase Activity in Cultured Growth Plate Chondrocytes in a Maturation-Dependent Manner
Chondrocytes metabolize 25-(OH)D3 to the two active dihydroxylated forms of the secosteroid, 1,25-(OH)2D3 and 24,25-(OH)2D3. The aim of the present study was to examine the activity of the enzymes responsible fo...
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Article
Purification, Amino Acid Sequence, and cDNA Sequence of a Novel Calcium-Precipitating Proteolipid Involved in Calcification of Corynebacterium matruchotii
Corynebacterium matruchotii is a microbial inhabitant of the oral cavity associated with dental calculus formation. It produces membrane-associated proteolipid capable of inducing hydrox...
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Article
The effects of 17\-estradiol on chondrocyte differentiation are modulated by vitamin D3 metabolites
Both 17β-estradiol (17β) and the vitamin D metabolites, 1,25-(OH)2D3 (1,25) and 24,25-(OH)2D3 (24,25), regulate endochondral bone formation in vivo and in vitro. The effects of 17β are sex-specific and cell matur...
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Article
24,25-(OH)2D3 Regulation of Matrix Vesicle Protein Kinase C Occurs Both During Biosynthesis and in the Extracellular Matrix
Plasma membranes and matrix vesicles isolated from rat costochondral resting zone chondrocyte cultures contain predominantly protein kinase C alpha (PKCα) and PKCζ, respectively, and the level of PKC specific...
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Article
Evidence for the Involvement of Carbonic Anhydrase and Urease in Calcium Carbonate Formation in the Gravity-Sensing Organ of Aplysia californica
To better understand the mechanisms that could modulate the formation of otoconia, calcium carbonate granules in the inner ear of vertebrate species, we examined statoconia formation in the gravity-sensing or...
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Article
Matrix Vesicles Promote Mineralization in a Gelatin Gel
Extracellular matrix vesicles (MVs) are associated with initial calcification in a variety of tissues, but the mechanisms by which they promote mineralization are not certain. In this study, MVs isolated from ...
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Article
Vitamin D Metabolites Regulate Matrix Vesicle Metalloproteinase Content in a Cell Maturation-Dependent Manner
Matrix vesicles are extracellular organelles produced by cells that mineralize their matrix. They contain enzymes that are associated with calcification and are regulated by vitamin D metabolites in a cell mat...
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Article
Carbonic anhydrase is required for statoconia homeostasis in organ cultures of statocysts from Aplysia californica
A novel organ culture system has been developed to study the regulation of statoconia production in the gravity sensing organ in Aplysia californica. Statocysts were cultured in Leibovitz (L15) medium supplemente...
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Article
Dexamethasone promotes von kossa-positive nodule formation and increased alkaline phosphatase activity in costochondral chondrocyte cultures
This study examined the effect of dexamethasone on von Kossa-positive nodule formation and alkaline phosphate specific activity of costochondral chondrocytes at two distinct stages of maturation. The nodules f...
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Article
Matrix vesicles produced by osteoblast-like cells in culture become significantly enriched in proteoglycan-degrading metalloproteinases after addition of β-Glycerophosphate and ascorbic acid
Matrix vesicles, media vesicles, and plasma membranes from three well-characterized, osteoblast-like cells (ROS 17/2.8, MG-63, and MC-3T3-E1) were evaluated for their content of enzymes capable of processing t...
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Article
Modulation of matrix vesicle enzyme activity and phosphatidylserine content by ceramic implant materials during endosteal bone healing
This study examined effects of bone bonding and nonbonding implants on parameters associated with matrix vesicle-mediated primary bone formation, matrix vesicle alkaline phosphatase and phospholipase A2 specific ...
