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Article
Light-Induced Changes in Phytohormone Levels of Develo** Sunflower (Helianthus annuus L.) Seedlings
Daily changes in the content of phytohormones accumulating in the hypocotyls of young sunflower seedlings that grow under a 14/10-h light-to-dark (LD) photoperiod were followed using LC–MS. The objective of th...
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Article
Diurnal rhythmicity of endogenous phytohormones and phototropic bending capacity in potato (Solanum tuberosum L.) shoot cultures
Taking advantage of advanced high performance liquid chromatography–electrospray tandem–mass spectrometry (HPLC–ESI–MS/MS), we screened daily changes in concentrations of endogenous phytohormones of in vitro g...
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Article
Open AccessInteraction with gravitropism, reversibility and lateral movements of phototropically stimulated potato shoots
Phototropic (PT) and gravitropic (GT) bending are the two major tropic movements that determine the spatial position of potato shoots. We studied PT bending of potato plantlets grown under long-day photoperiod...
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Article
Erratum to: Daily Changes in the Competence for Photo- and Gravitropic Response by Potato Plantlets
Competence for phototropic (PT) and gravitropic (GT) bending by potato plantlets grown in vitro manifests regular daily changes indicating possible involvement of circadian regulation. Unilateral stimulation w...
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Article
Daily Changes in the Competence for Photo- and Gravitropic Response by Potato Plantlets
Competence for phototropic (PT) and gravitropic (GT) bending by potato plantlets grown in vitro manifests regular daily changes indicating possible involvement of circadian regulation. Unilateral stimulation o...
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Article
Variability in shoot cultures regenerated from hairy roots of Gentiana punctata
Differences among three clones of Gentiana punctata L. hairy root shoot regenerants were investigated in relation to their growth patterns, production of secondary metabolites and 2D protein profiles. Prominent d...
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Article
Hairy roots formation in recalcitrant-to-transform plant Chenopodium rubrum
Susceptibility of C. rubrum to Agrobacterium-mediated transformation was demonstrated by inoculating the petioles of in vitro grown plants with A. rhizogenes strain A4M70GUS. Hairy roots were produced in 8 % of e...
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Article
Somatic embryogenesis and plant regeneration from root sections of Allium schoenoprasum L.
A protocol has been developed for somatic embryogenesis and subsequent plant regeneration in Allium schoenoprasum L. Calli were induced from root sections isolated from axenic seedlings and cultivated on media co...
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Article
Effects of donor plant environmental conditions on immature embryo cultures derived from worldwide origin wheat genotypes
The influence of donor plant growth conditions in years 2003, 2004, and 2005 on tissue culture response (TCR) traits of 96 wheat genotypes was evaluated. Immature embryos, collected 12–15 days after anthesis f...
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Article
Propagation and xanthone content of Gentianella austriaca shoot cultures
Shoot cultures of Gentianella austriaca (A. & J. Kerner) Dostal established from seedling epicotyls were maintained on MS medium supplemented with 2.22 μM BA and 0.54 μM NAA. A characteristic feature of these cul...
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Article
Nickel tolerance and hyperaccumulation in shoot cultures regenerated from hairy root cultures of Alyssum murale Waldst et Kit
Shoot cultures of nickel hyperaccumulating Alyssum murale were established from epicotyl explants of seedlings aseptically germinated on hormone-free MS medium. They were further maintained on media with 0–0.92 μ...
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Article
Secondary somatic embryogenesis versus caulogenesis from somatic embryos of Aesculus carnea Hayne.: developmental stage impact
Somatic embryos of red horse chestnut, derived from cultures maintained through repetitive somatic embryogenesis for a few years, were subjected to induction of secondary regeneration. The embryos were divided...
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Article
In vitro propagation of Chimonanthus praecox (L.), a winter flowering ornamental shrub
A procedure for the micropropagation of Chimonanthus praecox (L) Link, wintersweet, has been developed using buds from adult trees excised in spring. Shoot cultures established on Murashige and Skoog (1962) mediu...
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Article
Effects of plant genotype and bacterial strain on Agrobacterium tumefaciens-mediated transformation of Brassica oleracea L. var. capitata
Two inbred lines of Brassica oleracea L. var. capitata were transformed with two Agrobacterium tumefaciens strains harboring resistance to herbicide Basta: AGL1/pDM805 and LBA4404/pGKB5 (LB5-1). Inoculated cotyle...
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Article
Shoot and root culture of Hypericum perforatum L. transformed with Agrobacterium rhizogenes A4M70GUS
Hairy root cultures of Hypericum perforatum were obtained following inoculation of aseptically germinated seedlings with A. rhizogenes strain A4M70GUS. Effect of sucrose on the growth and biomass production of ha...
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Article
Agrobacterium rhizogenes-mediated transformation of Brassica oleracea var. sabauda and B. oleracea var. capitata
Agrobacterium rhizogenes A4M70GUS-mediated transformation of Savoy cabbage (Brassica oleracea L. var. sabauda) and two local lines of cabbage (B. oleracea L. var. capitata) was obtained u...
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Article
Nickel hyperaccumulation in shoot cultures of Alyssum markgrafii
Shoot cultures of Alyssum markgrafii O.E. Shulz, endemic nickel hyperaccumulating species of central Balkan, were established and maintained on Murashige and Skoog medium supplemented with 0.2 mg dm−3 benzyladeni...
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Article
Introduction of Resistance to Herbicide Basta® in Savoy Cabbage
Resistance to herbicide Basta® was introduced into pure inbred lines of Savoy cabbage (Brassica oleracea L. var. sabauda) by cocultivation of cotyledon and hypocotyl explants with Agrobacterium tumefaciens strain...
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Article
Propagation of Chelidonium majus L. by Somatic Embryogenesis
Direct somatic embryogenesis in celandine (Chelidonium majus L.) was achieved in epicotyl explants of seedlings after prolonged cultivation on Murashige and Skoog medium with or without plant growth regulators. S...
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Article
Effect of Irradiance, Sugars and Nitrogen on Leaf Size of In Vitro Grown Ceratonia Siliqua L.
Carob (Ceratonia siliqua L.) has compound pinnate leaves consisting of 4 – 6 pairs of leaflets. However, in conditions of in vitro culture only one pair of leaflets develops. With increasing irradiance from 9.3 t...
