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Chapter
EPO in Burns
Erythropoietin (EPO), dessen Existenz bereits vor mehr als einem Jahrhundert postuliert worden ist, ist ein körpereigenes Hormon, welches abhängig vom Sauerstoffpartialdruck durch Fibroblasten des Nierenparenc...
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Chapter
Regenerative Therapies
Skin is by far the largest organ in the human body. Its surface ranges between 1.5 and 1.8 m2 and the thickness varies between 0.5 (lower eyelid) and 15 mm (foot sole) in a young average adult, resulting in a tis...
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Article
Open AccessA multi-center study on the regenerative effects of erythropoietin in burn and scalding injuries: study protocol for a randomized controlled trial
Although it was initially assumed that erythropoietin (EPO) was a hormone that only affected erythropoiesis, it has now been proposed that EPO plays an additional key role in the regulation of acute and chroni...
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Article
Paracetamol treatment increases telomerase activity in rat embryonic liver cells
Although paracetamol is known to have a damaging effect, this pharmaceutical is widely applied to pregnant and lactating women. Despite substantial progress in our understanding of its hepatotoxicity, some mec...
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Article
Open AccessDetermination of pore size distribution at the cell-hydrogel interface
Analyses of the pore size distribution in 3D matrices such as the cell-hydrogel interface are very useful when studying changes and modifications produced as a result of cellular growth and proliferation withi...
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Chapter
Skin
Skin is by far the largest organ in the human body. Its surface ranges between 1.5 and 1.8 m² and the thickness varies between 0.5 (lower eyelid) and 10 mm (foot sole) in a young average adult, resulting in a ...
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Article
Cryopreservation of primary porcine liver cells in an organotypical sandwich model in a clinically relevant flat membrane bioreactor
To overcome the logistical difficulties of continuously supplying freshly-isolated, primary porcine liver cells to bioartificial liver support bioreactors, we developed a cryopreservation method using an organ...
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Article
Open AccessBiomechanical modulation of collagen fragment-induced anabolic and catabolic activities in chondrocyte/agarose constructs
The present study examined the effect of collagen fragments on anabolic and catabolic activities by chondrocyte/agarose constructs subjected to dynamic compression.
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Article
Telomerase activity and hepatic functions of rat embryonic liver progenitor cell in nanoscaffold-coated model bioreactor
Presently, there is growing interest on telomerase activity in all cells (somatic cells, stem cells, cancerous cells and others) since this activity is associated with cellular changes such as proliferation, d...
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Article
Analysis of stem cell lipids by offline HPTLC-MALDI-TOF MS
MALDI-TOF MS is traditionally used for “proteomics”, but is also a useful tool for lipid analysis. Depending on the applied matrix, however, some lipid classes are more sensitively detected than other ones and...
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Article
Von der Gewebezüchtung zur individuellen Geweberegeneration – Der Mensch als Bioreaktor
Für den Menschen – als der zentralen individuellen organischen Entität – ist die Regeneration ein biologischer Prozess der Reparatur und Erneuerung; die Prävention in all ihren Eigenschaften ist das umfassende...
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Article
Cartilage tissue engineering and bioreactor systems for the cultivation and stimulation of chondrocytes
Damage to and degeneration of articular cartilage is a major health issue in industrialized nations. Articular cartilage has a particularly limited capacity for auto regeneration. At present, there is no estab...
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Article
Intracellular nucleotide pools and ratios as tools for monitoring dedifferentiation of primary porcine hepatocytes in culture
The effect of two culture configurations (single collagen gel and double collagen gel) and of two hormones (insulin and glucagon) on the differentiated status and the intracellular nucleotide pools of primary ...
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Article
Present and Future Developments in Hepatic Tissue Engineering for Liver Support Systems
The liver is the most important organ for the biotransformation of xenobiotics, and the failure to treat acute or acute-on-chronic liver failure causes high mortality rates in affected patients. Due to the lac...
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Chapter
Bioreactor Developments for Tissue Engineering Applications by the Example of the Bioartificial Liver
Tissue engineering is the application of the principles and methods of engineering and the life sciences towards the development of biological substitutes to restore, maintain or improve functions. It is an ar...
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Article
Diazepam metabolism and albumin secretion of porcine hepatocytes in collagen-sandwich after cryopreservation
Cryopreserved porcine hepatocytes in collagen cultures secreted albumin (up to 98 ± 3 μg ml−1; non-cryopreserved controls: 100 ± 9 μg ml−1) and metabolised diazepam (67.5% ± 7.5% of initially applied diazepam are...
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Chapter
Performance of a flat membrane bioreactor utilizing porcine hepatocytes cultured in an extracellular matrix
To be clinically successful, an extracorporeal bioartificial liver (BAL) should allow maintenance of liver specific functions in vitro and achievement of high density cell culture for minimising the size of the B...
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Chapter
Engineering of a miniature 3-D hepatic cell plate culture model
The development of primary liver cell culture techniques has a long standing history that has contributed significantly to our current knowledge about the relevance of individual aspects of the hepatic microen...
