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Chapter
Commercial Production of Biohydrogen Using Microbes
Biohydrogen is a clean fuel, which can be produced through direct or indirect biophotolysis, photo-fermentation, and dark fermentation by microorganisms. In dark fermentation, organic waste degradation and hyd...
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Chapter
Biomedical and Pharmaceutical Applications of Xylan and Its Derivatives
Hemicelluloses are non-soluble plant cell wall polysaccharides that can be separated by alkali or hydrolyzed by dilute acids. Major hemicelluloses are xylan, glucuronoxylan, arabinoxylan, mannan, glucomannan, ...
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Chapter
Thermostable Enzymes from Clostridium thermocellum
The production of bioenergy from wastes attracts worldwide attention to overcome energy crisis and increasing pollution (Thakur et al., Microbial fermentation and enzyme technology, Taylor and Francis Group, B...
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Chapter
Biogas Biorefinery
Biogas is a gaseous fuel comprising methane and carbon dioxide as major constituents. It is produced by microbial consortium of acidogenic, acetogenic and methanogenic bacteria in the absence of oxygen from th...
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Article
Deciphering the mode of action, structural and biochemical analysis of heparinase II/III (PsPL12a) a new member of family 12 polysaccharide lyase from Pseudopedobacter saltans
Heparinases are widely used for production of clinically and therapeutically important bioactive oligosaccharides and in analyzing the polydisperse, heterogeneous, and complex structures of heparin/heparan sul...
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Article
Rye bran as fermentation matrix boosts in situ dextran production by Weissella confusa compared to wheat bran
The consumption of fiber-rich foods such as cereal bran is highly recommended due to its beneficial health effects. Pre-fermentation of bran with lactic acid bacteria can be used to improve the otherwise impai...
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Article
Isolation, purification and functional characterization of glucansucrase from probiotic Lactobacillus plantarum DM5
The probiotic Lactobacillus plantarum DM5 isolated from fermented beverage Marcha of Sikkim was explored for its ability to produce extracellular glucansucrase during fermentation. The strain L. plantarum DM5 dis...
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Article
Mannan specific family 35 carbohydrate-binding module (CtCBM35) of Clostridium thermocellum: structure analysis and ligand binding
The three-dimensional model of the CtCBM35 (Cthe 2811), i.e. the family 35 carbohydrate binding module (CBM) from the Clostridium thermocellum family 26 glycoside hydrolase (GH) β-mannanase, generated by Modeller...
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Article
Probiotic Potential of Pediococcus pentosaceus CRAG3: A New Isolate from Fermented Cucumber
A novel strain of lactic acid bacterium isolated from fermented cucumber was selected due to its high glucansucrase activity. It was identified on the basis of 16S rRNA sequence analysis as Pediococcus pentosace...
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Article
Anti-listerial Bactericidal Activity of Lactobacillus plantarum DM5 Isolated from Fermented Beverage Marcha
The strain Lactobacillus plantarum DM5 was isolated from fermented beverage Marcha of Sikkim and explored for its antagonistic activity against food-borne pathogens. The cell-free supernatant of L. plantarum DM5 ...
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Article
Evolving Roles of Probiotics in Cancer Prophylaxis and Therapy
In nutraceutical science, the ingestible live microbes ‘probiotics’ are regarded for their ability to confer multiplicity of health benefits on the consumers. Wide spectrum impact of these friendly microbes on...
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Article
Immobilization of glucansucrase for the production of gluco-oligosaccharides from Leuconostoc mesenteroides
Glucansucrase from Leuconostoc mesenteroides was immobilized in 1 % (w/v) with sodium alginate to produce oligosaccharides. Glucansucrase gave three activity bands of approx. 240, 178, and 165 kDa after periodic ...
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Article
Potentials of Exopolysaccharides from Lactic Acid Bacteria
Recent research in the area of importance of microbes has revealed the immense industrial potential of exopolysaccharides and their derivative oligosaccharides from lactic acid bacteria. However, due to lack o...
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Chapter
Lactic Acid Bacteria in Food Industry
Lactic acid bacteria (LAB) are known through ages for their wide applications in food, pharmaceutical and chemical industries. But recently LAB have aroused interest for their ability to secrete extracellular...
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Article
Functional oligosaccharides: production, properties and applications
Worldwide interest in oligosaccharides has been increasing ever since they were accorded the prebiotic status. The oligosaccharides of various origin like, bacteria, algae, fungi and higher plants have been us...
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Article
Stabilization of dextransucrase from Leuconostoc mesenteroides NRRL B-640
Stabilization of dextransucrase from Leuconostoc mesenteroides NRRL B-640 with various stabilizers at different temperatures was studied. Dextransucrase was stable at lower temperatures (10–30°C) and lost the act...
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Article
Basidiobolomycosis of the Nose and Face: A Case Report and a Mini-Review of Unusual Cases of Basidiobolomycosis
Subcutaneous zygomycosis is a chronic infection caused by fungus of the order Entomophthorales. It can have varying presentations and presents in the nose and face area with gradually progressing subcutaneous ...
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Article
Enhanced production of a novel dextran fromLeuconostoc mesenteroides NRRL B-640 by Response Surface Methodology
In our earlier study dextran produced byLeuconostoc mesenteroides NRRL B-640 was reported to possess novel food gelling and thickening properties (Puramaet al., 2009). In the present study response surface method...
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Article
Structural and biochemical properties of lichenase from Clostridium thermocellum
The recombinant enzyme lichenase of size 30 kDa was over-expressed using E. coli cells and purified by immobilized metal ion affinity chromatography (IMAC) and size exclusion chromatography. The enzyme displayed ...
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Article
Recombinant lichenase fromClostridium thermocellum binds glucomannan but not to lichenan: Analysis by affinity electrophoresis
A family 26, glycoside hydrolase (GH26) named lichenase of the molecular size 30 kDa fromClostridium thermocellum previously cloned into an expression vector pET21a was over-expressed inEscherichia coli BL-21 cel...