18 Result(s)
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Article
Osmoregulation in Dunaliella tertiolecta: effects of salt stress, and the external pH on the internal pH
The intracellular pH of the halotolerant green algae Dunaliella tertiolecta, was determined by the distribution of 5,5-dimethyl-2(14C)-oxalolidine-2,5-dione (DMO) between the cell and the surrounding medium. 5,5-...
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Article
Ed Tolbert and his love for science: A journey from sheep ranch continuesċċċ
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Article
Application of Response Surface Methodology for Maximizing Dextransucrase Production from Leuconostoc mesenteroides NRRL B-640 in a Bioreactor
The production of dextransucrase from Leuconostoc mesenteroides NRRL B-640 was investigated using statistical approaches. Plackett–Burman design with six variables, viz. sucrose, yeast extract, K2HPO4, peptone, b...
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Article
Functional oligosaccharides: production, properties and applications
Worldwide interest in oligosaccharides has been increasing ever since they were accorded the prebiotic status. The oligosaccharides of various origin like, bacteria, algae, fungi and higher plants have been us...
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Article
Bioethanol Production Involving Recombinant C. thermocellum Hydrolytic Hemicellulase and Fermentative Microbes
The enhancement of the biomass productivity of Escherichia coli cells harbouring the truncated 903 bp gene designated as glycoside hydrolase family 43 (GH43) from Clostridium thermocellum showing hemicellulase ac...
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Article
Immobilization of glucansucrase for the production of gluco-oligosaccharides from Leuconostoc mesenteroides
Glucansucrase from Leuconostoc mesenteroides was immobilized in 1 % (w/v) with sodium alginate to produce oligosaccharides. Glucansucrase gave three activity bands of approx. 240, 178, and 165 kDa after periodic ...
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Living Reference Work Entry In depth
Dextran and Food Application
An increasing consumer trend towards healthy and additive-free food has made dextran from food grade lactic acid bacteria (LAB) an attractive solution. Dextrans are homopolysaccharides of d-glucose produced by ex...
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Article
In silico structural characterization and molecular docking studies of first glucuronoxylan-xylanohydrolase (Xyn30A) from family 30 glycosyl hydrolase (GH30) from Clostridium thermocellum
CtXynGH30 is a carbohydrate active modular enzyme and component of cellulosome of Clostridium thermocellum. The full length CtXynGH30 contains an N-terminal catalytic module named as Xyn3...
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Reference Work Entry In depth
Dextran and Food Application
An increasing consumer trend towards healthy and additive-free food has made dextran from food grade lactic acid bacteria (LAB) an attractive solution. Dextrans are homopolysaccharides of d-glucose produced by ex...
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Article
Recovery and Purification of Oligosaccharides from Copra Meal by Recombinant Endo-β-mannanase and Deciphering Molecular Mechanism Involved and Its Role as Potent Therapeutic Agent
Production of manno-oligosaccharides (MOSs) from pretreated and defatted copra meal (dFCO) hydrolysis was achieved by endo-mannanase. Structural characterization of dFCO by FT-IR and NMR exhibited resemblance ...
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Article
A New Member of Family 11 Polysaccharide Lyase, Rhamnogalacturonan Lyase (CtRGLf) from Clostridium thermocellum
A thermostable, alkaline rhamnogalacturonan lyase (RG lyase) CtRGLf, of family 11 polysaccharide lyase from Clostridium thermocellum was cloned, expressed, purified and biochemically characterised. Both, the full...
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Article
Molecular Cloning, Expression and Characterization of Pectin Methylesterase (CtPME) from Clostridium thermocellum
Many phytopathogenic micro-organisms such as bacteria and fungi produce pectin methylesterases (PME) during plant invasion. Plants and insects also produce PME to degrade plant cell wall. In the present study,...
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Article
Dextran Utilization During Its Synthesis by Weissella cibaria RBA12 Can Be Overcome by Fed-Batch Fermentation in a Bioreactor
Weissella cibaria RBA12 produced a maximum of 9 mg/ml dextran (with 90% efficiency) using shake flask culture under the optimized concentration of medium components viz. 2% (w/v) of each sucrose,...
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Article
Molecular Cloning, Expression and Biochemical Characterization of a Family 5 Glycoside Hydrolase First Endo-Mannanase (RfGH5_7) from Ruminococcus flavefaciens FD-1 v3
The cellulosomal enzyme, RfGH51/2, of Ruminococcus flavefaciens contains an N-terminal module, a family 5 glycoside hydrolase GH5_4 with a putative endoglucanase activity, while C-terminal domain is a putative en...
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Article
Molecular Characterization, Regioselective and Synergistic Action of First Recombinant Type III α-L-arabinofuranosidase of Family 43 Glycoside Hydrolase (PsGH43_12) from Pseudopedobacter saltans
α-l-Arabinofuranosidase (PsGH43_12) of family 43 glycoside hydrolase and subfamily 12 from Pseudopedobacter saltans was cloned, over-expressed and biochemically characterized. PsGH43_12 displayed molecular mass, ...
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Chapter
Emerging Trends in Novel Technological Advancements in Oligosaccharide Production and Their Potential Applications as Prebiotics with Beneficial Effects
In the fast-growing global population, several health problems have arisen owing to bad lifestyles and consumption of unhealthy food. Prebiotics are a boon to reversing these ill effects and benefitting human ...
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Chapter
Enzymes of Industrial Significance and Their Applications
Enzymes play an enormous role in enriching and saving various forms of life on earth including humans. Enzymes also called biological catalysts have immense applications in various industrial sectors such as b...
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Chapter
Fundamentals and Industrial Applications of Modern Genetic Engineering
Biotechnological innovations have become crucial for the growth of three major industrial sectors of economy: fuel, pharmaceuticals and food. Genetic engineering utilizes various molecular biological technique...
