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    Chapter

    Optimization of the Firefly Luciferase Reaction for Analytical Purposes

    The optimization of assays has two purposes: (1) to increase the sensitivity of the assay so that low levels of the analyte can be determined; and (2) to prevent small changes of the reaction conditions from h...

    Arne Lundin in Bioluminescence: Fundamentals and Applications in Biotechnology - Volume 2 (2014)

  2. Article

    Open Access

    Isotopic resonance at 370 ppm deuterium negatively affects kinetics of luciferin oxidation by luciferase

    Since 1930s, it has been known that some biochemical and biological processes exhibit abnormal kinetics at a deuterium concentration in the local environment of 250–600 ppm, which is 2–4 times higher that the ...

    Sergey Rodin, Paola Rebellato, Arne Lundin, Roman A. Zubarev in Scientific Reports (2018)