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Chapter
Optimization of the Firefly Luciferase Reaction for Analytical Purposes
The optimization of assays has two purposes: (1) to increase the sensitivity of the assay so that low levels of the analyte can be determined; and (2) to prevent small changes of the reaction conditions from h...
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Article
Open AccessIsotopic resonance at 370 ppm deuterium negatively affects kinetics of luciferin oxidation by luciferase
Since 1930s, it has been known that some biochemical and biological processes exhibit abnormal kinetics at a deuterium concentration in the local environment of 250–600 ppm, which is 2–4 times higher that the ...