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Article
Open AccessProteomic associations with forced expiratory volume: a Mendelian randomisation study
A decline in forced expiratory volume (FEV1) is a hallmark of respiratory diseases that are an important cause of morbidity among the elderly. While some data exist on biomarkers that are related to FEV1, we s...
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Article
Open AccessEndoBind detects endogenous protein-protein interactions in real time
We present two high-throughput compatible methods to detect the interaction of ectopically expressed (RT-Bind) or endogenously tagged (EndoBind) proteins of interest. Both approaches provide temporal evaluatio...
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Article
Structure-guided combination therapy to potently improve the function of mutant CFTRs
Available corrector drugs are unable to effectively rescue the folding defects of CFTR-ΔF508 (or CFTR-F508del), the most common disease-causing mutation of the cystic fibrosis transmembrane conductance regula...
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Article
The Hippo kinases LATS1 and 2 control human breast cell fate via crosstalk with ERα
Ablation of the large tumour suppressor kinases 1 and 2 promotes a luminal breast cell phenotype through stabilization of oestrogen receptor-α, thereby changing human breast cell fate.
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Article
Open AccessIdentification of synthetic lethality of PRKDC in MYC-dependent human cancers by pooled shRNA screening
MYC family members are among the most frequently deregulated oncogenes in human cancers, yet direct therapeutic targeting of MYC in cancer has been challenging thus far. Synthetic lethality provides an opportu...
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Article
High-content analysis of three-dimensional tumor spheroids: investigating signaling pathways using small hairpin RNA
In the past, creating three-dimensional (3D) tumor spheroids that were suitable for high-throughput screening (HTS) was a difficult and often expensive process. We describe how to couple easy, controllable 3D ...
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Article
A functional genomics approach to the mode of action of apratoxin A
The cyanobacterial metabolite apratoxin A (1) demonstrates potent cytotoxicity against tumor cell lines by a hitherto unknown mechanism. We have used functional genomics to elucidate the molecular basis for this ...
