-
Protocol
Isolation and Culture of Murine Primary Chondrocytes: Costal and Growth Plate Cartilage
Primary chondrocyte isolation and culture is a useful tool to characterize how cellular perturbations impact chondrocyte behavior and mineralization in vitro. This protocol conveys methods for isolating and cu...
-
Protocol
Demineralized Murine Skeletal Histology
Cartilage and bone are specialized skeletal tissues composed of unique extracellular matrices. Bone, in particular, has a highly calcified or mineralized matrix that makes microtomy and standard histological s...
-
Article
Open AccessNotch signaling controls chondrocyte hypertrophy via indirect regulation of Sox9
RBPjk-dependent Notch signaling regulates both the onset of chondrocyte hypertrophy and the progression to terminal chondrocyte maturation during endochondral ossification. It has been suggested that Notch sig...
-
Protocol
Isolation and Culture of Murine Primary Chondrocytes
To identify factors that are necessary and sufficient for chondrocyte hypertrophic differentiation and cartilage matrix mineralization, primary chondrocyte culture models have been developed. Here we describe ...
-
Article
Open Accessβ-catenin/cyclin D1 mediated development of suture mesenchyme in calvarial morphogenesis
Mouse genetic study has demonstrated that Axin2 is essential for calvarial development and disease. Haploid deficiency of β-catenin alleviates the calvarial phenotype caused by Axin2 deficiency. This loss-of-f...
