-
Article
Open AccessCardiomyocyte precursors generated by direct reprogramming and molecular beacon selection attenuate ventricular remodeling after experimental myocardial infarction
Direct cardiac reprogramming is currently being investigated for the generation of cells with a true cardiomyocyte (CM) phenotype. Based on the original approach of cardiac transcription factor-induced reprogr...
-
Article
Open AccessAn inactivating human TRPC6 channel mutation without focal segmental glomerulosclerosis
Transient receptor potential cation channel-6 (TRPC6) gene mutations cause familial focal segmental glomerulosclerosis (FSGS), which is inherited as an autosomal dominant disease. In patients with TRPC6-related F...
-
Article
Open AccessHuman Cell Atlas and cell-type authentication for regenerative medicine
In modern biology, the correct identification of cell types is required for the developmental study of tissues and organs and the production of functional cells for cell therapies and disease modeling. For dec...
-
Article
Open AccessUpregulated immuno-modulator PD-L1 in malignant peripheral nerve sheath tumors provides a potential biomarker and a therapeutic target
Malignant peripheral nerve sheath tumors (MPNSTs) are rare aggressive sarcomas with poor prognosis. More than half of MPNSTs develop from benign precursor tumors associated with neurofibromatosis type 1 (NF1) ...
-
Chapter
Naturwissenschaftliche Grundlagen im Kontext einer klinischen Anwendung von humanen induzierten pluripotenten Stammzellen
Humane induzierte pluripotente Stammzellen (hiPS-Zellen) können patienten- und krankheitsspezifisch aus nahezu jeder Zelle des Körpers erzeugt werden und besitzen Eigenschaften, die eigentlich nur Zellen aus e...
-
Article
Open AccessThe TreaT-Assay: A Novel Urine-Derived Donor Kidney Cell-Based Assay for Prediction of Kidney Transplantation Outcome
Donor-reactive immunity plays a major role in rejection after kidney transplantation, but analysis of donor-reactive T-cells is not applied routinely. However, it has been shown that this could help to identif...
-
Protocol
Generating Multiple Kidney Progenitors and Cell Types from Human Pluripotent Stem Cells
Human pluripotent stem cells (hPSCs) have been well known for their ability to generate kidney cell types. We developed a protocol that utilizes a set of growth factors to give rise to kidney progenitors, whic...
-
Article
Parallel generation of easily selectable multiple nephronal cell types from human pluripotent stem cells
Human pluripotent stem cells (hPSCs) provide a source for the generation of defined kidney cells and renal organoids applicable in regenerative medicine, disease modeling, and drug screening. These application...
-
Article
Open AccessEffector T cell subclasses associate with tumor burden in neurofibromatosis type 1 patients
Neurofibromatosis type 1 (NF1) is a hereditary tumor syndrome caused by mutations of the NF1 gene and resulting dysregulation of the Ras-pathway. In addition to peripheral nerve tumors, affected tissues include t...
-
Chapter and Conference Paper
A Method for an Integrated Software-Based Virtualisation of System Inputs
The influence of the consumer electronic industry to the automotive industry leads to shortened development times for the automotive domain. To keep up with the competitors and to enable the customer maximum c...
-
Article
Selective cell targeting and lineage tracing of human induced pluripotent stem cells using recombinant avian retroviruses
Human induced pluripotent stem cells (hiPSC) differentiate into multiple cell types. Selective cell targeting is often needed for analyzing gene function by overexpressing proteins in a distinct population of ...
-
Article
Open AccessMGFM: a novel tool for detection of tissue and cell specific marker genes from microarray gene expression data
Identification of marker genes associated with a specific tissue/cell type is a fundamental challenge in genetic and cell research. Marker genes are of great importance for determining cell identity, and for u...
-
Article
Human adipose-Derived Mesenchymal Stem Cells Improve Motor Functions and are Neuroprotective in the 6-Hydroxydopamine-Rat Model for Parkinson’s Disease when Cultured in Monolayer Cultures but Suppress Hippocampal Neurogenesis and Hippocampal Memory Function when Cultured in Spheroids
Adult human adipose-derived mesenchymal stem cells (MSC) have been reported to induce neuroprotective effects in models for Parkinson´s disease (PD). However, these effects strongly depend on the most optimal ...
-
Article
Open AccessComparative computational analysis of pluripotency in human and mouse stem cells
Pluripotent cells can be subdivided into two distinct states, the naïve and the primed state, the latter being further advanced on the path of differentiation. There are substantial differences in the regulati...
-
Article
Effects of pigment epithelium derived factor (PEDF) on malignant peripheral nerve sheath tumours (MPNSTs)
Neurofibromatosis type 1 (NF1) is an inherited genetic disease affecting 1 in 3,500 individuals. A prominent feature of NF1 is the formation of benign tumours of the peripheral nerve sheath (neurofibromas). Ho...
-
Article
Open AccessUsing ontologies to study cell transitions
Understanding, modelling and influencing the transition between different states of cells, be it reprogramming of somatic cells to pluripotency or trans-differentiation between cells, is a hot topic in current...
-
Article
Open AccessImpact of heart failure on the behavior of human neonatal stem cells in vitro
Clinical cardiac cell therapy using autologous somatic stem cells is restricted by age and disease-associated impairment of stem cell function. Juvenile cells possibly represent a more potent alternative, but ...
-
Article
Open AccessCELDA - an ontology for the comprehensive representation of cells in complex systems
The need for detailed description and modeling of cells drives the continuous generation of large and diverse datasets. Unfortunately, there exists no systematic and comprehensive way to organize these dataset...
-
Article
Open AccessSerum biomarkers for neurofibromatosis type 1 and early detection of malignant peripheral nerve-sheath tumors
Neurofibromatosis type 1 (NF1) is a hereditary tumor syndrome characterized by the development of benign nerve-sheath tumors, which transform to malignant peripheral nerve-sheath tumors (MPNST) in about 8 to 1...
-
Article
Open AccessScope and Impact of International Research in Human Pluripotent Stem Cells
In a recent study published in this journal it was claimed that the rate of publications from US-based authors in the human embryonic stem cell (hESC) research field was slowing or even declining from 2008 to ...
