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  1. No Access

    Article

    Functional analysis of single enzymes combining programmable molecular circuits with droplet-based microfluidics

    The analysis of proteins at the single-molecule level reveals heterogeneous behaviours that are masked in ensemble-averaged techniques. The digital quantification of enzymes traditionally involves the observat...

    Guillaume Gines, Rocίo Espada, Adèle Dramé-Maigné… in Nature Nanotechnology (2024)

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    Article

    Toggling Between Two Limit Cycles in a Molecular Ecosystem

    In this article, we introduce an oscillatory molecular system with two independent limit cycles, dependent on initial conditions. The system is composed of two crossed Predator-Prey oscillators, in which the p...

    Adrien Fauste-Gay, Nicolas Lobato-Dauzier, Alexandre Baccouche… in New Generation Computing (2022)

  3. No Access

    Article

    Massively parallel and multiparameter titration of biochemical assays with droplet microfluidics

    Titration of the optimal ratios of multicomponent biochemical reactions can be a laborious task. This protocol describes how to explore ∼10,000 combinations of concentrations of one to three reaction component...

    Alexandre Baccouche, Shu Okumura, Rémi Sieskind, Elia Henry… in Nature Protocols (2017)