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Article
Open AccessProceedings of the Frontiers of Retrovirology Conference 2016
Oral presentations
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Article
Integrase-mediated spacer acquisition during CRISPR–Cas adaptive immunity
Bacteria and archaea insert spacer sequences acquired from foreign DNAs into CRISPR loci to generate immunological memory. The Escherichia coli Cas1–Cas2 complex mediates spacer acquisition in vivo, but the molec...
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Article
Open AccessThe mechanism of H171T resistance reveals the importance of Nδ-protonated His171 for the binding of allosteric inhibitor BI-D to HIV-1 integrase
Allosteric HIV-1 integrase (IN) inhibitors (ALLINIs) are an important new class of anti-HIV-1 agents. ALLINIs bind at the IN catalytic core domain (CCD) dimer interface occupying the principal binding pocket o...
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Article
Open AccessHost and viral determinants for MxB restriction of HIV-1 infection
Interferon-induced cellular proteins play important roles in the host response against viral infection. The Mx family of dynamin-like GTPases, which include MxA and MxB, target a wide variety of viruses. Despi...
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Article
The structural biology of HIV-1: mechanistic and therapeutic insights
HIV-1 replication relies on the proper functioning of specific viral proteins, three of which — protease, integrase, and reverse transcriptase with associated ...
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Article
Open AccessHIV-1 integrase SUMOylation and viral replication
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Article
Retroviral intasome assembly and inhibition of DNA strand transfer
Integrase is an essential retroviral enzyme that binds both termini of linear viral DNA and inserts them into a host cell chromosome. The structure of full-length retroviral integrase, either separately or in ...
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Article
Open AccessBiochemical and virological analysis of the 18-residue C-terminal tail of HIV-1 integrase
The 18 residue tail abutting the SH3 fold that comprises the heart of the C-terminal domain is the only part of HIV-1 integrase yet to be visualized by structural biology. To ascertain the role of the tail reg...
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Protocol
Isolation and Analysis of HIV-1 Preintegration Complexes
A discerning feature of the retrovirus lifecycle is the covalent integration of the viral reverse transcript into a chromosome within the infected cell. Integration is required for productive infection and the...
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Article
Solution structure of the HIV-1 integrase-binding domain in LEDGF/p75
Lens epithelium-derived growth factor (LEDGF)/p75 is the dominant binding partner of HIV-1 integrase (IN) in human cells. We have determined the NMR structure of the integrase-binding domain (IBD) in LEDGF and...
