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Article
Circulating DNA reveals a specific and higher fragmentation of the Y chromosome
Chromosome stability is a key point in genome evolution, particularly that of the Y chromosome. The Y chromosome loss in blood and tumor cells is well established. Through processes that are common to other ch...
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Article
Open AccessA straightforward method to quantify circulating mRNAs as biomarkers of colorectal cancer
Optimizing the biomarker combination to be analyzed in liquid biopsies should improve personalized medicine. We developed a method to purify circulating cell-free mRNAs from plasma samples and to quantify them...
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Article
Open AccessNeutrophil extracellular traps have auto-catabolic activity and produce mononucleosome-associated circulating DNA
As circulating DNA (cirDNA) is mainly detected as mononucleosome-associated circulating DNA (mono-N cirDNA) in blood, apoptosis has until now been considered as the main source of cirDNA. The mechanism of cirD...
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Article
Open AccessGuidelines for pre-analytical conditions for assessing the methylation of circulating cell-free DNA
Methylation analysis of circulating cell-free DNA (cirDNA), as a liquid biopsy, has a significant potential to advance the detection, prognosis, and treatment of cancer, as well as many genetic disorders. The ...
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Article
Reply to Comment on: ‘Hypoxia differently modulates the release of mitochondrial and nuclear DNA’
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Article
Open AccessTowards systematic nomenclature for cell-free DNA
Cell-free DNA (cfDNA) has become widely recognized as a promising candidate biomarker for minimally invasive characterization of various genomic disorders and other clinical scenarios. However, among the obsta...
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Article
Does the newly observed inflammatory syndrome in children demonstrate a link between uncontrolled neutrophil extracellular traps formation and COVID-19?
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Article
Open AccessHypoxia differently modulates the release of mitochondrial and nuclear DNA
We investigated the influence of hypoxia on the concentration of mitochondrial and nuclear cell-free DNA (McfDNA and NcfDNA, respectively).
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Article
Open AccessQuantifying circulating cell-free DNA in humans
To our knowledge, this is the first comprehensive study on the influence of several pre-analytical and demographic parameters that could be a source of variability in the quantification of nuclear and mitochon...
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Article
Open AccessNew insights into structural features and optimal detection of circulating tumor DNA determined by single-strand DNA analysis
Circulating cell-free DNA (cfDNA) has received increasing interest as an apparent breakthrough approach in diagnostics, personalized medicine, and tumor biology. However, the structural features of cfDNA are p...
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Protocol
A Targeted Q-PCR-Based Method for Point Mutation Testing by Analyzing Circulating DNA for Cancer Management Care
Circulating cell-free DNA (cfDNA) is a valuable source of tumor material available with a simple blood sampling enabling a noninvasive quantitative and qualitative analysis of the tumor genome. cfDNA is releas...
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Article
Is antisense an appropriate nomenclature or design for oligodeoxynucleotides aimed at the inhibition of HIV-1 replication?
We have evaluated the specificity and the variation in activity against human immunodeficiency virus (HIV) infection of antisense oligodeoxynucleotides (ODNs) with regard to factors such as dose-response range...
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Chapter
Pre-analytical Requirements for Analyzing Nucleic Acids from Blood
Circulating nucleic acids have received an increasing scrutiny over the past decade with some applications, such as in prenatal diagnosis and oncology, being on the verge of use in clinical practice. It is cru...
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Chapter
Detection of Genetic Alterations by Nucleic Acid Analysis: Use of PCR and Mass Spectroscopy-Based Methods
Cell free circulating DNA, isolated from blood has emerged as a potential biomarker in oncology. There has been also considerable progress towards theranostic application of circulating DNA. These applications...
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Chapter
Circulating DNA and miRNA Isolation
Analysis of circulating nucleic acids undoubtedly represents a breakthrough in the diagnostic field and in predictive, preventive and personalized medicine. In order to adequately and systematically study and ...
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Article
Clinical validation of the detection of KRAS and BRAF mutations from circulating tumor DNA
Alain Thierry and his colleagues offer a new allele-specific, quantitative PCR–based method designed for the detection of point mutations and determination of mutation load using circulating cell-free DNA isol...
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Article
Open AccessComputing with bacterial constituents, cells and populations: from bioputing to bactoputing
The relevance of biological materials and processes to computing—alias bioputing—has been explored for decades. These materials include DNA, RNA and proteins, while the processes include t...
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Article
Open AccessA rapid microwell fluorescence immunoassay for cellular protein detection
In this paper, we describe a simple, rapid, specific, sensitive, and reliable method, the FICP method (Fluorescence Immunoassay for Cellular Protein detection) which is readily applicable to the detection of p...
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Article
Characterization of a Synthetic Anionic Vector for Oligonucleotide Delivery Using in Vivo Whole Body Dynamic Imaging
Purpose. To compare the pharmacokinetics and bioavailability of an oligonucleotide delivered in a free form or using cationic or anionic synthetic carrier systems.
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Article
Kaposi's sarcoma in pregnant women
