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Article
Open AccessDual proteomics of infected macrophages reveal bacterial and host players involved in the Francisella intracellular life cycle and cell to cell dissemination by merocytophagy
Bacterial pathogens adapt and replicate within host cells, while host cells develop mechanisms to eliminate them. Using a dual proteomic approach, we characterized the intra-macrophage proteome of the facultat...
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Article
Open AccessAirway environment drives the selection of quorum sensing mutants and promote Staphylococcus aureus chronic lifestyle
Staphylococcus aureus is a predominant cause of chronic lung infections. While the airway environment is rich in highly sialylated mucins, the interaction of S. aureus with sialic acid is poorly characterized. Us...
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Article
Open AccessFulminant arterial vasculitis as an unusual complication of disseminated staphylococcal disease due to the emerging CC1 methicillin-susceptible Staphylococcus aureus clone: a case report
Staphylococcus aureus has emerged as a leading cause of invasive severe diseases with a high rate of morbidity and mortality worldwide. The wide spectrum of clinical manifestations and outcome observed in staphyl...
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Chapter
Francisella tularensis: Causative Agent of Tularemia and Biothreat Agent
The bacterium Francisella tularensis subspecies tularensis (also designated F. tularensis type A) is a potential biological warfare agent, classified as Tier 1 Select Agent by the US Centers for Disease Control a...
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Article
Open AccessThe metabolic enzyme fructose-1,6-bisphosphate aldolase acts as a transcriptional regulator in pathogenic Francisella
The enzyme fructose-bisphosphate aldolase occupies a central position in glycolysis and gluconeogenesis pathways. Beyo...
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Article
Open AccessA widespread family of polymorphic toxins encoded by temperate phages
Polymorphic toxins (PTs) are multi-domain bacterial exotoxins belonging to distinct families that share common features in terms of domain organization. PTs are found in all major bacterial clades, including m...
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Protocol
Detection of the Interaction Between Host and Bacterial Proteins: Eukaryotic Nucleolin Interacts with Francisella Elongation Factor Tu
Dissecting the interaction between bacterial and host proteins is fundamental in understanding pathogenesis. It is also very helpful for exploring new therapeutic approaches, either preventive or curative. Her...
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Article
Open AccessIdentification of small RNAs in Francisella tularensis
Regulation of bacterial gene expression by small RNAs (sRNAs) have proved to be important for many biological processes. Francisella tularensis is a highly pathogenic Gram-negative bacterium that causes the disea...
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Article
Open AccessA novel receptor – ligand pathway for entry of Francisella tularensis in monocyte-like THP-1 cells: interaction between surface nucleolin and bacterial elongation factor Tu
Francisella tularensis, the causative agent of tularemia, is one of the most infectious human bacterial pathogens. It is phagocytosed by immune cells, such as monocytes and macrophages. The precise mechanisms tha...
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Chapter
The Maltose B Region in Salmonella Typhimurium, Escherichia Coli and Other Enterobacteriaceae
The envelope of Gram negative bacteria plays a critical role in the primary interactions between bacteria and their environment. Such interactions include the sensing of the presence of substrates (chemotaxis)...
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Article
DNA sequence analysis of the lamB gene from Klebsiella pneumoniae: implications for the topology and the pore functions in maltoporin
We have determined the sequence of the lamB gene from Klebsiella pneumoniae. It encodes the precursor to the LamB protein, a 429 amino acid polypeptide with maltoporin function. Comparison with the Escherichia co...
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Article
High efficiency transformation of Salmonella typhimurium and Salmonella typhi by electroporation
Salmonella typhimurium and S. typhi were transformd with high efficiency by electroporation. Transformation efficiencies of up to 1010 transformants per μg of pBR322 were obtained. In contrast to chemical transf...
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Article
Expression of Human β1 and β2 Adrenergic Receptors in E. Coli As a New Tool for Ligand Screening
E. coli transformed with appropriate plasmid vectors express human β1 and β2-adrenergic receptors that retain the binding properties of the corresponding receptors in mammalian tissues. The experimental procedure...
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Article
Mutagenesis by random linker insertion into the lamB gene of Escherichia coli K12
Gene lamB encodes an outer membrane protein involved in maltose and maltodextrin transport as well as phage adsorption. The active form is a trimer. We characterized 11 mutations in lamB, obtained after random in...
