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Article
Open AccessDirect photoresponsive inhibition of a p53-like transcription activation domain in PIF3 by Arabidopsis phytochrome B
Photoactivated phytochrome B (PHYB) binds to antagonistically acting PHYTOCHROME-INTERACTING transcription FACTORs (PIFs) to regulate hundreds of light responsive genes in Arabidopsis by promoting PIF degradation...
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Article
Open AccessAn optogenetic system to control membrane phospholipid asymmetry through flippase activation in budding yeast
Lipid asymmetry in biological membranes is essential for various cell functions, such as cell polarity, cytokinesis, and apoptosis. P4-ATPases (flippases) are involved in the generation of such asymmetry. In Sacc...
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Article
Open AccessMechanism of early light signaling by the carboxy-terminal output module of Arabidopsis phytochrome B
Plant phytochromes are thought to transduce light signals by mediating the degradation of phytochrome-interacting transcription factors (PIFs) through the N-terminal photosensory module, while the C-terminal m...
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Article
Root hydrotropism is controlled via a cortex-specific growth mechanism
Plants can acclimate by using tropisms to link the direction of growth to environmental conditions. Hydrotropism allows roots to forage for water, a process known to depend on abscisic acid (ABA) but whose mol...
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Article
Tissue-specific regulation of flowering by photoreceptors
Plants use various kinds of environmental signals to adjust the timing of the transition from the vegetative to reproductive phase (flowering). Since flowering at the appropriate time is crucial for plant repr...
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Article
Open AccessInhibition of arabidopsis hypocotyl elongation by jasmonates is enhanced under red light in phytochrome B dependent manner
Jasmonates are phytohormones derived from oxygenated fatty acids that regulate a broad range of plant defense and developmental processes. In Arabidopsis, hypocotyl elongation under various light conditions wa...
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Chapter
Functions of Different Domains of Phytochrome
Phytochrome is a photoreceptor regulating various aspects of plant photomorphogenesis. Phytochrome affects plant development by regulating gene expression. Recent analysis using the DNA microarray technique re...
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Article
Dimers of the N-terminal domain of phytochrome B are functional in the nucleus
A plant modulates its developmental processes in response to light by several informational photoreceptors such as phytochrome. Phytochrome is a dimeric chromoprotein which regulates various aspects of plant d...
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Protocol
Light-Induced Nuclear Targeting of PhytochromeB-sGreen Fluorescent Protein in Plants
Phytochrome is a ubiquitous plant photoreceptor, that regulates various aspects of plant development (1). Phytochrome is a soluble chromoprotein of ∼120 kDa, to which a linear tetrapyrrole chromophore is covalent...
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Article
Spatial distribution of phytochromes
Phytochromes are chromoproteins which mediate several light responses in plants. Phytochrome proteins are encoded by a gene family which is currently being characterized in several plant species. Analysis of t...
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Article
Over-expression of a C-terminal region of phytochrome B
In an attempt to identify domains directly involved in the signal transduction of phytochrome B (phyB), we over-expressed the achromophoric C-terminal half of phyB under control of the CaMV-35S promoter in tra...
