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  1. No Access

    Article

    Engineering of geranylgeranyl pyrophosphate synthase levels and physiological conditions for enhanced carotenoid and astaxanthin synthesis in Xanthophyllomyces dendrorhous

    The basidiomycetous yeast, Xanthophyllomyces dendrorhous, is one of the very few organisms which can be used for biological production of the carotenoid astaxanthin. crtE cDNA has been cloned from this fungus for...

    Jürgen Breitenbach, Hans Visser, Jan C. Verdoes… in Biotechnology Letters (2011)

  2. No Access

    Article

    Genes involved in carotene synthesis and mating in Blakeslea trispora

    Mating of Blakeslea trispora and other molds of the order Mucorales requires the interaction of mycelia of opposite sex, (+) and (−), leading to the development of specialized structures and to an enhanced accumu...

    Vera Kuzina, Humberto Ramírez-Medina, Hans Visser… in Current Genetics (2008)

  3. Article

    Open Access

    Genome sequencing and analysis of the versatile cell factory Aspergillus niger CBS 513.88

    The filamentous fungus Aspergillus niger is widely exploited by the fermentation industry for the production of enzymes and organic acids, particularly citric acid. We sequenced the 33.9-megabase genome of A. nig...

    Herman J Pel, Johannes H de Winde, David B Archer, Paul S Dyer… in Nature Biotechnology (2007)

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  4. No Access

    Article

    Characterisation of the Aspergillus niger dapB gene, which encodes a novel fungal type IV dipeptidyl aminopeptidase

    We have cloned the Aspergillus niger dapB gene. Analysis of its nucleotide sequence and the corresponding protein sequence indicates that the gene encodes a type IV dipeptidyl aminopeptidase (DPP IV). Based upon ...

    Ruud Jalving, Jeroen Godefrooij, Wim J. ter Veen… in Molecular Genetics and Genomics (2005)

  5. No Access

    Article

    Characterisation of Aspergillus niger prolyl aminopeptidase

    We have cloned a gene (papA) that encodes a prolyl aminopeptidase from Aspergillus niger. Homologous genes are present in the genomes of the Eurotiales A. nidulans, A. fumigatus and Talaromyces emersonii, but the...

    Daniëlle E. J. W. Basten, Antoine P. H. A. Moers… in Molecular Genetics and Genomics (2005)

  6. No Access

    Chapter

    Fermentation and Carotenoid Analysis of the Yeast Xanthophyllomyces dendrorhous [Phaffia rhodozyma)

    To study the effect of growth conditions on the production of carotenoids byX. dendrorhous.

    Hans Visser, Jan C. Verdoes… in Non-Conventional Yeasts in Genetics, Bioch… (2003)

  7. No Access

    Chapter

    Metabolic Engineering of the Carotenoid Biosynthetic Pathway in Xanthophyllomyces dendrorhous [Phaffia rhodozyma)

    To study the effect of overexpression of the phytoene desaturase encoding gene (crtl) on carotenoid production in Xanthophyllomyces dendrorhous.

    Jan C. Verdoes, Hans Visser… in Non-Conventional Yeasts in Genetics, Bioch… (2003)

  8. No Access

    Article

    Cloning, characterization and heterologous expression of epoxide hydrolase-encoding cDNA sequences from yeasts belonging to the genera Rhodotorula and Rhodosporidium

    Epoxide hydrolase-encoding cDNA sequences were isolated from the basidiomycetous yeast species Rhodosporidium toruloides CBS 349, Rhodosporidium toruloides CBS 14 and Rhodotorula araucariae CBS 6031 in order to e...

    Hans Visser, Carel A.G.M. Weijers, Albert J.J. van Ooyen… in Biotechnology Letters (2002)

  9. No Access

    Article

    Codon usage in Xanthophyllomyces dendrorhous (formerly Phaffia rhodozyma)

    By sequence analysis of 96 randomly selected clones in a cDNA library of Xanthophyllomyces dendrorhous, ten novel, full-length clones encoding cytoplasmic ribosomal proteins (rp) were found. The deduced amino aci...

    Jan C. Verdoes, Albert J.J. van Ooyen in Biotechnology Letters (2000)

  10. No Access

    Article

    Efficient Transformation of the Astaxanthin-Producing Yeast Phaffia Rhodozyma

    An efficient transformation system for the astaxanthin-producing yeast Phaffia rhodozyma was developed based on electroporation that routinely yields approximately 1000 transformants per μg of plasmid DNA. The...

    Jan Wery, Jan C. Verdoes, Albert J.J. van Ooyen in Biotechnology Techniques (1998)

  11. No Access

    Article

    Phytase-containing Transgenic Seeds as a Novel Feed Additive for Improved Phosphorus Utilization

    Phytate is the main storage form of phosphorus in many plant seeds, but bound in this form it is a poor nutrient for monogastric animals. Its availability can be significantly increased by addition of the enzy...

    Jan Pen, Theo C. Verwoerd, Peter A. van Paridon, Rob F. Beudeker… in Bio/Technology (1993)

  12. No Access

    Article

    The yeastKluyveromyces lactis as an efficient host for heterologous gene expression

    Several different yeast species have been developed into systems for efficient heterologous gene expression. In this paper we review foreign gene expression in the dairy yeastKluyveromyces lactis. This yeast pres...

    Bart W. Swinkels, Albert J. J. van Ooyen, Fons J. Bonekamp in Antonie van Leeuwenhoek (1993)

  13. No Access

    Article

    Direct screening for high-level expression of an introduced α-amylase gene in plants

    A method is described for obtaining transgenic plants with a high level of expression of the introduced gene. Tobacco protoplasts were transformed with an expression construct containing a translational fusion...

    Jan Pen, Albert J. J. van Ooyen, Peter J. M. van den Elzen… in Plant Molecular Biology (1992)

  14. No Access

    Article

    Production of Active Bacillus licheniformis Alpha-Amylase in Tobacco and its Application in Starch Liquefaction

    As a first example of the feasibility of producing industrial bulk enzymes in plants, we have expressed Bacillus licheniformis α-amylase in transgenic tobacco, and applied the seeds directly in starch liquificati...

    Jan Pen, Lucy Molendijk, Wim J. Quax, Peter C. Sijmons… in Bio/Technology (1992)

  15. No Access

    Article

    Kluyveromyces as a Host for Heterologous Gene Expression: Expression and Secretion of Prochymosin

    We have developed the yeast Kluyveromyces lactis as a host organism for the production of the milk-clotting enzyme chymosin. In contrast to Saccharomyces cerevisiae, we found that this yeast is capable of the syn...

    Johan A. van den Berg, Kees J. van der Laken, Albert J. J. van Ooyen… in Bio/Technology (1990)

  16. No Access

    Article

    Long terminal repeats of endogenous mouse mammary tumour virus contain a long open reading frame which extends into adjacent sequences

    The integrated DNA copies (proviruses) of RNA tumour virus genomes are flanked by long terminal repeat (LTR) sequences, which are generated by the duplication of segments present at both ends of the viral RNA1. L...

    Nick Kennedy, Gudrun Knedlitschek, Bernd Groner, Nancy E. Hynes, Peter Herrlich… in Nature (1982)

  17. No Access

    Article

    In vitro transcription of three different ribosomal RNA cistrons of E. coli; heterogeneity of control regions

    Ribosomal RNA synthesis from three different rRNA cistrons of E. coli, located on different phage DNAs was compared and found to have the same characteristics as regards chain length, salt and temperature depende...

    Ben A. Oostra, Albert J. J. van Ooyen, Max Gruber in Molecular and General Genetics MGG (1977)

  18. No Access

    Article

    Specific inhibition of ribosomal RNA synthesis in vitro by guanosine 3′ diphosphate, 5′ diphosphate

    CONSIBERABLE evidence has accumulated that guanosine 3′-diphosphate, 5′-diphosphate (ppGpp)1 is involved in the regulation of ribosomal RNA (rRNA) synthesis in Escherichia coli in vivo (see ref. 2). The involveme...

    ALBERT J. J. VAN OOYEN, HERMAN A. DE BOER, GEERT AB, MAX GRUBER in Nature (1975)