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Article
Tritium-Probe Method in a Study of Adsorption Layers of Lysozyme on the Surface of Detonation Nanodiamonds
Tritium-labeled lysozyme was used to determine its adsorption on the surface of nanodiamonds produced by detonation synthesis. It was found that the amount of the adsorbed protein depends on the charge of the ...
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Article
Characterization of Tobacco Mosaic Virus Virions and Repolymerized Coat Protein Aggregates in Solution by Small-Angle X-Ray Scattering
The structure of tobacco mosaic virus (TMV) virions and stacked disk aggregates of TMV coat protein (CP) in solution was analyzed by synchrotron-based small-angle X-ray scattering (SAXS) and negative contrast ...
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Article
Influence of Carbon Material Supports on the Efficiency of the Isotope Exchange between Dalargine and Tritium
Isotope exchange between dalargine applied onto various supports [glass, activated carbon, few-layer graphite (FLG)] and molecular tritium, performed with activation on a tungsten wire and on 5% Pd/C, 10% Pd/C...
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Article
Structure of Potato Virus A Coat Protein Particles and Their Dissociation
This paper reports on a complex structural analysis of the potato virus A coat protein using a set of complementary physico-chemical methods. We have demonstrated previously that this protein does not exist as...
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Article
Removal of Antimicrobial Peptides from Aqueous Solutions Using Carbon Nanotubes
The aim of this work is to study the possibility of removing antimicrobial peptides arenicin-1 and tachyplesin-1 from aqueous solutions using new highly hydrophobic nanomaterials—carbon nanotubes (CNTs) Taunit...
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Article
Analysis of free amino acids in mammalian brain extracts
An optimized method for analysis of free amino acids using a modified lithium-citrate buffer system with a Hitachi L-8800 amino acid analyzer is described. It demonstrates clear advantages over the sodium-citr...
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Article
Thiamine induces long-term changes in amino acid profiles and activities of 2-oxoglutarate and 2-oxoadipate dehydrogenases in rat brain
Molecular mechanisms of long-term changes in brain metabolism after thiamine administration (single i.p. injection, 400 mg/kg) were investigated. Protocols for discrimination of the activities of the thiamine ...
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Article
Structural properties of potexvirus coat proteins detected by optical methods
It has been shown by X-ray analysis that cores of coat proteins (CPs) from three potexviruses, flexible helical RNA-containing plant viruses, have similar α-helical structure. However, this similarity cannot e...
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Article
Investigation of the complex antibiotic INA-5812
A concentrate with the antimicrobial activity has been isolated from the culture broth of Streptomyces roseoflavus INA-Ac-5812. Its further fractionation by reversed-phase HPLC has resulted in six fractions. It h...
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Article
Solid phase isotope exchange of deuterium and tritium for hydrogen in human recombinant insulin
Reaction of a high-temperature solid-phase catalytic isotope exchange in peptides and proteins under the action of the catalytically activated spillover hydrogen was studied. The reaction of human recombinant ...
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Article
Tritium planigraphy: Differences in the spatial structures of the influenza virus M1 protein in crystal, solution, and virion
The structure of the M1 protein of the influenza virus A/Puerto Rico/8/34 (PR8, subtype H1N1) in solution at acidic pH and in the composition of the virion has been studied by the tritium planigraphy method. A...
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Article
Intrinsically unstructured regions in the C domain of the influenza virus M1 protein
The M1 matrix protein of the influenza virus is one of the main structural components of the virion that performs several different functions in the infected cell. X-ray analysis (with 2.08 Å resolution) has b...
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Article
Estimation of the evolutionary stability of the Influenza A virus: Prediction of variable regions in the domain structure of the M1 protein
Influenza virus is a human pathogen that is responsible for several pandemics with high death rates. Two programs were written for the statistical analysis of the coding regions of genes from extensive samples...
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Article
Quantitation of the glycoprotein spike area on the surface of enveloped viruses
The density of glycoprotein (GP) distribution on the virion surface substantially influences the virus infectivity and pathogenicity. A method to quantitatively determine the area occupied by surface GP spikes...
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Article
Nonequilibrium processes in reactions of hot tritium atoms with cooled solid targets. Influence of the atomizer temperature on formation of labeled substances
A system consisting of a cold target and “hot” atoms generated by dissociation of tritium on a tungsten wire was studied with the aim to determine conditions for preparing tritium-labeled organic compounds wit...
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Article
Determination of concentration and aggregate size in influenza virus preparations from true UV absorption spectra
Light scattering is known to make a considerable contribution to ultraviolet absorption spectra of influenza virus (Flu) preparations. We applied extrapolation to analysis of this contribution. Ultraviolet spe...
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Article
Comparative Study of Reaction of Atomic Tritium with Glucosamine and Amino Acids
Reaction of amino acids (glycine and serine) and amino sugar (glucosamine) with atomic tritium generated by thermal dissociation of molecular tritium on a tungsten filament was studied. A frozen aqueous soluti...
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Article
Bacteriorhodopsin. Correspondence of the photocycle and electrogenesis with sites of the molecule
Correspondence of phases of electrogenesis, photocycle transitions, and proton transfer with the proton transporting groups of bacteriorhodopsin was studied. The structure of bacteriorhodopsin was considered b...
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Article
Bacteriorhodopsin. Correspondence of the photocycle and electrogenesis with sites of the molecule
Correspondence of phases of electrogenesis, photocycle transitions, and proton transfer with the proton transporting groups of bacteriorhodopsin was studied. The structure of bacteriorhodopsin was considered b...
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Article
Conformational Differences between Native and Recombinant Horseradish Peroxidase Revealed by Tritium Planigraphy
Significant conformational differences between native and recombinant horseradish peroxidase have been shown by tritium planigraphy, which includes a method of thermal activation of tritium followed by amino a...
