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Article
Expression and use of the green fluorescent protein as a reporter system in Legionella pneumophila
The gene encoding the green fluorescent protein (GFP) was used as a reporter gene in Legionella pneumophila. To analyze GFP expression in Legionella, transcriptional fusions of gfp with the Legionella-specific m...
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Article
Differential expression of Listeria monocytogenes virulence genes in mammalian host cells
We have used RT-PCR and GFP-mediated fluorescence to analyse the regulation of PrfA-dependent virulence genes of Listeria monocytogenes during proliferation in mammalian host cells. Our data show that most of th...
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Article
The gene cluster inlC2DE of Listeria monocytogenes contains additional new internalin genes and is important for virulence in mice
In this work we identified and characterized a gene cluster containing three internalin genes of Listeria monocytogenes EGD. These genes, termed inlG, inlH and inlE, encode proteins of 490, 548 and 499 amino aci...
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Article
The Listeria monocytogenesiap gene as an indicator gene for the study of PrfA-dependent regulation
The iap gene of Listeria monocytogenes encodes the extracellular protein p60, which possesses a murein hydrolase activity necessary for septum separation. We constructed L. monocytogenes EGD strains harbouring p...
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Article
Catalase-negativeListeria monocytogenes causing lethal sepsis and meningitis in an adult hematologic patient
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Article
Extracellular PagC-HlyAS fusion protein for the generation and identification of Salmonella-specific antibodies
The outer membrane protein, PagC, of Salmonella typhimurium was converted into a secreted protein by linking the 61-amino-acid long, C-terminal signal sequence of the E. coli hemolysin protein (HlyAS) to the mat...