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Chapter and Conference Paper
Substrate Water Oxygen Exchange in Photosystem II: Insights from Mutants and Ca vs. Sr Substitution
Oxygen-18 exchange kinetics have been conducted for three point mutants associated with the oxygen evolving core of PSII and for centers that have undergone biosynthetic Sr/Ca exchange. The mutants D1-D170H, D...
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Chapter
Characterisation and Functional Studies on a New Photosystem 2 Preparation from the Thermophilic Cyanobacterium Synechococcus Elongatus
A new Photosystem 2 (PS2) preparation from the thermophilic cyanobacterium Synechococcus elongatus was developed consisting in the detergent solubilisation of proteins from the thylakoid membrane and the purifica...
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Chapter
FTIR Study of TYRD and TYRZ: Hydrogen Bonding Interactions
The two redox active tyrosines of photosystem II (PS2), TyrD and TyrZ have been studied by FTIR difference spectroscopy. This study aims at a better understanding of the structural factors that explain their spec...
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Chapter
Tyrosine D Radical in Photosystem II Investigated by FTIR Difference Spectroscopy
Two redox-active tyrosine residues located on the D1 and D2 polypeptides of the photosystem II reaction center (PS II) have been extensively characterized by EPR spectroscopy. TyrZ (D1 161), which is involved in ...
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Chapter
Near Infrared Yb3+ Vibronic Sideband Spectroscopy of Proteins: Amino-Acid Side Chain Models
Recently, we have reported the use of near-infrared (NIR) Yb3+ vibronic sideband (VSB) spectroscopy to obtain structural information of metal-binding sites in proteins (1). In the NIR fluorescence spectrum of met...
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Article
The quenching characteristics of potassium iridic chloride and their meaning for the origin of chlorophyll fluorescence components
To understand the origins of the different lifetime components of photosystem 2 (PS2) chlorophyll (Chl) fluorescence we have studied their susceptibility to potassium iridic chloride (K2IrCl6) which has been show...
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Article
Mutagenesis by random cloning of an Escherichia coli kanamycin resistance gene into the genome of the cyanobacterium Synechocystis PCC 6803: Selection of mutants defective in photosynthesis
Photosynthetic mutants of the cyanobacterium Synechocystis PCC 6803 were produced by a random cartridge mutagenesis method leading to gene inactivation. This procedure relies on random ligation of an Escherichia ...