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Article
Establishment of an efficient regeneration system and in vitro polyploid induction based on the bulblet centre in Lilium rosthornii Diels
Lilium rosthornii Diels, commonly known as the Nanchuan lily in China, is a unique wild lily species with potential medicinal or ornamental value. The development and utilization are mainly restricted to its hig...
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Article
Open AccessA high-quality haplotype genome of Michelia alba DC reveals differences in methylation patterns and flower characteristics
Michelia alba DC is a highly valuable ornamental plant of the Magnoliaceae family. This evergreen tropical tree commonly grows in Southeast Asia and is adored for its delightful fragrance. Our study assembled the...
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Article
Open AccessSelf S-RNase reduces the expression of two pollen-specific COBRA genes to inhibit pollen tube growth in pear
Due to self-incompatibility (SI) prevents self-fertilization, natural or artificial cross-pollination has been conducted in many orchards to stabilize fruit yield. However, it is still puzzled which routes of ...
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Article
Development of an efficient micropropagation protocol for Nematanthus wettsteinii using leaf and shoot-tip explants
Nematanthus wettsteinii (Fritsch) H. E. Moore is one of the most popular ornamental species in China, and is known for its peculiar “goldfish” shape flower and is usually propagated by cuttings. This study aimed ...
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Article
Open AccessBBX7 interacts with BBX8 to accelerate flowering in chrysanthemum
The quantitative control of FLOWERING LOCUS T (FT) activation is important for the floral transition in flowering plants. However, the flowering regulation mechanisms in the day-neutral, summer-flowering chrys...
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Article
A stable method for Agrobacterium-mediated transformation of Mentha piperita and Mentha canadensis using internodal explants
Mentha species are planted worldwide for extraction of their metabolites, including essential oils and phenolic acid compounds, which have therapeutic and commercial value. Mentha piperita L. and Mentha canadensi...
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Article
Open AccessLSD 4.0: an improved database for comparative studies of leaf senescence
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Article
Overexpression of a putative 12-oxophytodienoate reductase gene, EpOPR1, enhances acetylshikonin production in Echium plantagineum
12-Oxophytodienoate reductase (OPR) enzymes in dicots are categorized as flavin mononucleotide (FMN)–dependent oxidoreductases and are divided into two subgroups. Although subgroup II OPRs have been identified...
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Article
Develo** a highly efficient regeneration system for leaves of tissue-cultured tetraploid Robinia pseudoacacia L.
We explored multiple experimental treatments for regenerating tissue-cultured shoots of tetraploid Robinia pseudoacacia L., and assessed the most suitable combination of treatments for the development of a highly...
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Article
Induction and early identification of tetraploid black locust by hypocotyl in vitro
Tetraploid black locust (Robinia pseudoacacia L.) has advantages over diploid varieties in growth and stress resistance. Previous studies had shown that tetraploid black locust can be induced only by treating sho...
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Article
Vitrification and proteomic analysis of embryogenic callus of Panax ginseng C. A. Meyer
Panax ginseng C. A. Meyer is a desirable medicinal plant due to its health benefits. With the growing interest in ginseng products, the safe preservation of genetically engineered material with unique attributes ...
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Article
Production of ginsenoside and polysaccharide by two-stage cultivation of Panax quinquefolium L. cells
Based on the results of carbon source consumption in cell suspension culture of Panax quinquefolium L., 30 g L−1 sucrose was fed into a 5-L stirred tank bioreactor on day 16 of culture to enhance cell density and...
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Article
Micropropagation and plant regeneration from embryogenic callus of Miscanthus sinensis
Miscanthus sinensis (Poaceae) is a typical perennial giant grass of East Asia. Due to its high photosynthetic efficiency, low input requirements, and high biomass production, M. sinensis shows ou...
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Article
Highly efficient plant regeneration and in vitro polyploid induction using hypocotyl explants from diploid mulberry (Morus multicaulis Poir.)
Efficient plant regeneration is essential for successful transformation and in vitro polyploidy induction in mulberry. A high frequency (80%) of plant regeneration from hypocotyls occurred under in vitro conditio...
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Article
Highly efficient embryo germination in vitro shortens the breeding cycle in Leymus chinensis
Chinese leymus is a perennial grass in the Gramineae family, which is widely distributed in Northern China and Mongolia. The breeding cycle in Chinese leymus is time-consuming because of its high level of seed do...
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Article
Development of transgenic rice pure lines with enhanced resistance to rice brown planthopper
Mature seed-derived callus from an elite Chinese japonica rice cv. Ewan 5 was cotransformed with two plasmids, pWRG1515 and pRSSGNAl, containing the selectable marker hygromycin phosphotransferase gene (hpt), the...
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Article
Cytogenetic characterization of 20 lymphoblastoid lines derived from human individuals differing in bleomycin sensitivity
Forty lymphoblastoid (lymphoid) lines were established from 42 volunteer blood donors, including healthy individuals and patients with head and neck carcinomas. Each peripheral blood sample was split into two ...
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Article
Production of monoclonal antibodies against avian LHRH-I
Production of antibodies against peptides or poorly antigenic proteins by conventional methods often requires either large quantities of the native immunogen or some chemical modification to increase their ant...
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Article
Early passage primate cell immortality is independent of tumorigenicity
Seven continuous primate cell lines were tested in three systems (nude mice, muscle organ culture, and soft agarose) for their ability to express characteristics usually associated with malignant cell lines. F...
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Article
Intracellular catabolin-like activity in cultured synovial tissue
The time course of the synthesis and secretion of catabolin-like activity by porcine synovial tissue was determined. Ex vivo tissue had no demonstrable activity, but short-term culture (<1 h) induced cellular ...