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In Vitro Culturing of Human Trophoblasts from Term Placenta
Since the early 1960s, researchers began culturing placental cells to establish an in vitro model to study the biology of human trophoblasts,...
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Isolation of total DNA from Placenta Samples, both Fresh and following Formalin and Paraffin Treatment
The isolation of DNA from placental tissue suspected of infection is an important tool for identifying microorganisms such as bacteria, fungi, and...
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Obtaining Tissues of Human Amniotic Membrane and Identification of Pluripotent Markers
The immunofluorescence technique has been used to identify pluripotent markers in the human amniotic epithelial cells (hAEC). hAEC belonging to human...
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Evaluation of Leukocyte Chemotaxis Induced by Human Fetal Membranes in an In Vitro Model
Leukocyte infiltration into the maternal-fetal interface is a consequence of the robust inflammation in the gestational tissues during term labor and...
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Immunophenoty** of Leukocytes in Amniotic Fluid
Immunophenoty** allows for the deep characterization of leukocytes present in biological samples. Here, we describe a complete procedure for the...
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Placental Trophoblast Cell Isolation from the Term Placenta
Multiple cell lines have been utilized over time in studying placental biology. Still, most of them rely on choriocarcinoma cells or immortalized...
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Alzheimer’s Disease Prevention and Treatment Based on Population-Based Approaches
The development of effective prevention and treatment strategies for Alzheimer’s disease (AD) and dementia is hindered by limited knowledge of the...
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CSF N-Glycomics Using MALDI MS Techniques
In this chapter, we will present the methodology currently applied in our laboratory for the structural elucidation of the cerebrospinal fluid (CSF)...
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CSF N-Glycomics Using High-Throughput UPLC-ESI Techniques in Alzheimer’s Disease
In this chapter, we will present a high-throughput method applied in our laboratory for the structural elucidation of the cerebrospinal fluid (CSF)...
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Detection of Intracellular Complement Activation by Nanoparticles in Human T Lymphocytes
The complement system is complex and includes two main components: the systemic or plasma complement and the so-called intracellular complement or...
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Detection of Induction of Mitochondrial Oxidative Stress by Nanoparticles in T Cells Using MitoSOX Red Dye
The induction of oxidative stress by engineered nanomaterials has been associated with cytotoxic and inflammatory responses, damaging healthy cells...
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Understanding the Role of Scavenger Receptor A1 in Nanoparticle Uptake by Murine Macrophages
Nanoparticles can be cleared from the circulation and taken up by tissue-resident macrophages. This property can be beneficial when drug or antigen...
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Measuring Size and Number Concentration of Metallic Nanoparticles Using spICP-MS
This protocol describes how to measure the size and concentration of individual metallic nanoparticles using inductively coupled plasma-mass...
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Methods for Analysis of Nanoparticle Immunosuppressive Properties
Adverse drug effects on immune system function represent a significant concern in the pharmaceutical industry, because 10–20% of drug withdrawal from...
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Evaluation of the Acute Anaphylactoid Reactogenicity of Nanoparticle-Containing Medicines and Vaccines Using the Porcine CARPA Model
A small fraction, up to 10%, of people treated intravenously with state-of-the-art nanoparticulate drugs or diagnostic agents develop an acute...
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Immunophenoty**, Part I: Instrument Calibration and Reagent Qualification for Immunophenoty** Analysis of Human Peripheral Blood Mononuclear Cell Cultures
Nanoparticles are increasingly used in biomedical applications to influence the way the immune system reacts to tumors and infectious disease-causing...
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Ion Quantitation in Liposomal Products Using RP-HPLC with Charged Aerosol Detection
Ion concentration in liposomal drugs is important for drug stability and drug release profile. However, quantifying ion concentration in liposomal...
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In Silico Models to Validate Novel Blood-Based Biomarkers
Biological validation of preliminary findings is a key prerequisite in biomarker discovery. In recent years, the development of advanced large-scale...
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Mass Spectrometry-Based Metabolomics Multi-platform for Alzheimer’s Disease Research
The integration of complementary analytical platforms is nowadays the most common strategy for comprehensive metabolomics analysis of complex...
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High-Throughput Lipidomic and Metabolomic Profiling for Brain Tissue and Biofluid Samples in Neurodegenerative Disorders
Recent research has revealed the potential of lipidomics and metabolomics in identifying new biomarkers and mechanistic insights for...