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Mass Spectrometry-Based Metabolomics Multi-platform for Alzheimer’s Disease Research

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Biomarkers for Alzheimer’s Disease Drug Development

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2785))

Abstract

The integration of complementary analytical platforms is nowadays the most common strategy for comprehensive metabolomics analysis of complex biological systems. In this chapter, we describe methods and tips for the application of a mass spectrometry multi-platform in Alzheimer’s disease research, based on the combination of direct mass spectrometry and orthogonal hyphenated approaches, namely, reversed-phase ultrahigh-performance liquid chromatography and gas chromatography. These procedures have been optimized for the analysis of multiple biological samples from human patients and transgenic animal models, including blood serum, various brain regions (e.g., hippocampus, cortex, cerebellum, striatum, olfactory bulbs), and other peripheral organs (e.g., liver, kidney, spleen, thymus).

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Authors and Affiliations

  1. Instituto de Investigación e Innovación Biomédica de Cádiz (INiBICA), Hospital Universitario Puerta del Mar, Universidad de Cádiz, Cádiz, Spain

    Álvaro González-Domínguez & Raúl González-Domínguez

  2. Department of Chemistry, Faculty of Experimental Sciences, University of Huelva, Huelva, Spain

    Ana Sayago & Ángeles Fernández-Recamales

  3. International Campus of Excellence CeiA3, University of Huelva, Huelva, Spain

    Ana Sayago & Ángeles Fernández-Recamales

Authors
  1. Álvaro González-Domínguez
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  2. Ana Sayago
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  3. Ángeles Fernández-Recamales
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  4. Raúl González-Domínguez
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Corresponding author

Correspondence to Raúl González-Domínguez .

Editor information

Editors and Affiliations

  1. Department of Psychiatry and Psychotherapy, LMU Klinikum, München, Germany

    Robert Perneczky

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González-Domínguez, Á., Sayago, A., Fernández-Recamales, Á., González-Domínguez, R. (2024). Mass Spectrometry-Based Metabolomics Multi-platform for Alzheimer’s Disease Research. In: Perneczky, R. (eds) Biomarkers for Alzheimer’s Disease Drug Development. Methods in Molecular Biology, vol 2785. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3774-6_6

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  • DOI: https://doi.org/10.1007/978-1-0716-3774-6_6

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3773-9

  • Online ISBN: 978-1-0716-3774-6

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