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Taxonomic Classification of Geminiviruses Based on Pairwise Sequence Comparisons
Geminiviridae is the largest and one of the most diverse families of plant viruses, comprising 14 genera demarcated based on host range, type of...
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Virus-Induced Heritable Gene Editing in Plants
Gene editing using clustered, regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) nuclease is an excellent tool for...
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Monitoring the Intracellular Trafficking of Virus-Induced Structures and Intercellular Spread of Viral Infection in Plants Using Endomembrane Trafficking Pathway-Specific Chemical Inhibitor and Organelle-Selective Fluorescence Dye
Infection by positive-strand RNA viruses induces extensive remodeling of the host endomembrane system in favor of viral replication and movement. The...
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Construction of Geminivirus Infectious Clones for Agroinoculation into Plants
The production of geminiviral infectious clones provides a standardized inoculum for use in several host–virus studies. Geminiviruses present either...
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Co-immunoprecipitation Assays to Detect Protein–Protein Interactions
Proteins usually do not function as monomers but rather perform their functions by interacting with themselves or other proteins....
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Synthetic Biology to Engineer Bacteriophage Genomes
Recent advances in the synthetic biology field have enabled the development of new molecular biology techniques used to build specialized...
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Rapid Bench to Bedside Therapeutic Bacteriophage Production
It has been over 100 years since bacteriophages (phages) were used as a human therapeutic. Since then, phage production has dramatically evolved....
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Nano/microformulations for Bacteriophage Delivery
Encapsulation methodologies allow the protection of bacteriophages for overcoming critical environmental conditions. Moreover, they improve the...
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A Review of Phage Therapy for Bone and Joint Infections
There is a strong rationale for using phages in patients with bone and joint infections (BJIs). Indeed, specific phages can infect and replicate in...
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Rescue of Infectious Salmon Anemia Virus (ISAV) from Cloned cDNA
The piscine orthomyxovirus called infectious salmon anemia virus (ISAV) is one of the most important emerging pathogens affecting the salmon industry...
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Reverse Genetics System for Rift Valley Fever Virus
Rift Valley fever virus (RVFV) is an important mosquito-borne virus that can cause severe disease manifestations in humans including ocular damage,...
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Detection of Plant Viruses Using Nanopore Sequencing Based Metagenomic Approach
Nanopore sequencing has proven to be a useful tool for the generic detection of plant viruses, especially in laboratories working with small number...
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Validation of High-Throughput Sequencing (HTS) for Routine Detection of Citrus Viruses and Viroids
The management of plant diseases relies on the accurate identification of pathogens that requires a robust and validated tool in terms of...
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Determination of the Mycovirome of a Necrotrophic Fungus
Next-generation sequencing (NGS) of total RNA has allowed the detection of novel viruses infecting different hosts, such as fungi, increasing our...
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Bacteriophage Treatment of Infected Diabetic Foot Ulcers
Diabetic foot ulcers occur as a common complication of diabetes. The concomitant infection significantly delays the healing of the ulcers. Antibiotic...
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The Oxford Nanopore MinION as a Versatile Technology for the Diagnosis and Characterization of Emerging Plant Viruses
The emergence of novel viral epidemics that could affect major crops represents a serious threat to global food security. The early and accurate...
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Discovery and Identification of Viruses Infecting Oomycetes
This chapter describes protocols suitable for the detection and identification of RNA viruses infecting oomycetes (so-called water molds of Kingdom...
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Bimolecular Fluorescence Complementation (BiFC) in Host–Virus Interactions
Bimolecular fluorescence complementation (BiFC) is an assay widely used for studying protein–protein interactions and determining the subcellular...
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Quantification of Plant Virus Seed Transmission Rate in Arabidopsis thaliana
More than 25% of all known plant viruses are transmitted through seeds, which makes this mode of dispersal of great importance for plant virus...