9 Result(s)
within
Protocol
Zhigang **
Life Sciences
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Protocol
Nucleotide Excision Repair in Saccharomyces cerevisiae Whole-Cell Extracts
Nucleotide excision repair (NER) is a particularly versatile pathway of DNA repair capable of removing a broad spectrum of DNA lesions in both prokaryotes and eukaryotes (1–3). NER involves steps of damage recogn...
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Protocol
Controlled Expression of Recombinant Genes and Preparation of Cell-Free Extracts in Yeast
Biochemistry is an important experimental tool in the study of protein functions. Biochemical studies frequently involve overexpression of a cloned gene and purification of the recombinant protein. The yeast Sacc...
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Protocol
Histone Deacetylase Activity Assay
Histone deacetylases (HDACs) are enzymes that catalyze the removal of acetyl groups from the ɛ-amino groups of conserved lysine residues in the amino terminal tail of histones. In humans, there are 18 potentia...
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Protocol
Manipulating Expression of Tonoplast Transporters
Plant vacuoles have multifaceted roles including turgor maintenance, cytosolic pH and ionic homeostasis, plant protection against environmental stress, detoxification, pigmentation, and cellular signaling. The...
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Protocol
Histone Deacetylase Activity Assay
Histone deacetylases (HDACs) are enzymes that catalyze the removal of acetyl groups from the ε-amino groups of conserved lysine residues in the amino terminal tail of histones. Accumulating evidence suggests t...
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Protocol
Novel Pollen Magnetofection System for Transformation of Cotton Plant with Magnetic Nanoparticles as Gene Carriers
Plant genetic transformation systems have facilitated insights into molecular plant biology and revolutionized commercial agriculture. Unfortunately, agrobacterium-mediated transformation was still the main me...
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Protocol
Biolistic DNA Delivery in Turfgrass Embryonic Callus Initiated from Mature Seeds
We describe a protocol for the establishment and preparation of cree** bentgrass (Agrostis stolonifera L.) cultivar “Penn A-4” embryonic calli, biolistic transformation, selection, and regeneration of transgeni...
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Protocol
Antibiotic Conditioning and Single Gavage Allows Stable Engraftment of Human Microbiota in Mice
Mice transplanted with human microbiota are essential tools for studying the role of microbiota in health and disease, striving for the development of microbiota-modulating therapeutics. Traditionally, germ-fr...
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Protocol
Genetic Screens for Floral Mutants in Arabidopsis thaliana: Enhancers and Suppressors
The flower is a hallmark feature that has contributed to the evolutionary success of land plants. Diverse mutagenic agents have been employed as a tool to genetically perturb flower development and identify ge...
