13,524 Result(s)
within
Life Sciences
Cell Biology
Yu** **
Protocol
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Protocol
Whole-Mount Immunofluorescence Staining to Visualize Cell Cycle Progression in Mouse Oocyte Meiosis
Whole-mount immunofluorescence allows direct visualization of the cellular architecture within cells. Here, we apply this technique to mouse oocytes to visualize spindle morphology and microtubule attachments ...
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Nuclear Transcription Factor Detection
Cellular fate is determined by the activity of nuclear transcription factors. Here, we describe a series of protocols for detecting transcription factors at both the transcript and protein levels in human adip...
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Imaging of Subcellular Distribution of Platinum in Single Cells Using Laser Ablation Inductively Coupled Plasma Mass Spectrometry
Laser ablation inductively coupled plasma-mass spectrometry (LA-ICP-MS) is a well-established and sensitive analytical technique, which provides high-resolution imaging of endogenous elements, element tagged-m...
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Assessing Cholesterol Efflux on Primary Human Trophoblast Cells
Cholesterol transport across the placenta must be tightly regulated to avoid a deficiency or an oversupply of cholesterol which is transferred from the mother to the fetus. In trophoblasts, the transport of ch...
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Quantifying ABCA1/apoA-1 Signaling Pathways with AFM Imaging and Lipidomic Analysis
The role of lipid metabolic pathways in the pathophysiology of primary open-angle glaucoma (POAG) has been thoroughly elucidated, with pathways involved in lipid-related disorders such as hypercholesterolemia ...
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Organotypic 3D Ex Vivo Co-culture Model of the Macro-metastasis/Organ Parenchyma Interface
The macro-metastasis/organ parenchyma interface (MMPI) is gaining increasing significance due to its prognostic relevance for cancer (brain) metastasis. We have developed an organotypic 3D ex vivo co-culture mode...
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Induced Formation of Plasma Membrane Protrusions with Porous Materials as Instructive Surfaces
The plasma membrane is a vital component in cellular processes, and its structure has a significant impact on cellular behavior. The physical characteristics of the extracellular environment, along with the pr...
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Proteomic Analysis of Dictyostelium discoideum by Mass Spectrometry
The large-scale proteomic analysis of Dictyostelium discoideum has contributed to our understanding of intracellular as well as secreted proteins in this versatile model eukaryote. Mass spectrometry-based proteom...
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Live-Imaging Centriole Amplification in Mouse Brain Multiciliated Cells
Multiciliated cells (MCC) display on their apical surface hundreds of beating cilia that propel physiological fluids. They line brain ventricles where they propel the cerebrospinal liquid, airways where they c...
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Quantitative Analysis of the Inactivation Process of Internalized Bacteria in Dictyostelium Cells
The understanding of the inactivation process of ingested bacteria by phagocytes is a key focus in the field of host-pathogen interactions. Dictyostelium is a model organism that has been at the forefront of unco...
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Nanoparticles and Their Applications in Lipid Signaling
This chapter provides an overview of the diverse range of applications associated with nanoparticles. The application of nanoparticles in the medical field has garnered considerable attention due to their uniq...
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Echocardiography to Assess Cardiac Structure and Function in Genetic Cardiomyopathies
Rodents are the most common experimental models used in cardiovascular research including studies of genetic cardiomyopathies. Genetic cardiomyopathies are characterized by changes in cardiac structure and fun...
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Studying Efferocytosis Dynamics in Tissue-Resident Macrophages Ex Vivo
In vitro cocultures of macrophages and apoptotic cells (ACs) provide a practical and useful tool to study efferocytosis. Here, we describe a method for automated quantification and imaging of recognition and e...
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Determination of the Crystal Structure of the Cell Wall-Anchored Proteins and Pilins
Surface proteins and pili (or pilus) anchored on the Gram-positive bacterial cell wall play a vital role in adhesion, colonization, biofilm formation, and immunomodulation. The pilus consists of building block...
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Adaptation of Human iPSC-Derived Macrophages Toward an Alveolar Macrophage-Like Phenotype Post-Intra-Pulmonary Transfer into Murine Models
Alveolar macrophages (AMs) represent crucial immune cells in the bronchioalveolar space of the . Given the important role in the host defense machinery and tissue homeostasis, AMs have been linked to a vari...
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Cell Cycle Map** Using Multiplexed Immunofluorescence
The development of technologies that allow measurement of the cell cycle at the level has revealed novel insights into the mechanisms that regulate cell cycle commitment and progression through DNA replicati...
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Single-Nucleus ATAC-seq for Map** Chromatin Accessibility in Individual Cells of Murine Hearts
During the last decade a wide range of single-cell and single-nucleus next-generation sequencing techniques have been developed, which revolutionized detection of rare cell populations, enabling creation of co...
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Real-Time Assessment of Mitochondrial Function in Cytotrophoblast and Syncytialized Trophoblast Cells Using the Seahorse XFe24 Extracellular Flux Analyzer
Physiological processes utilize variable amounts of energy required for optimal growth, development, and survival. This energy is supplied by total intracellular adenosine triphosphate (ATP) and is mainly gene...
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Double-Barrel Perfusion System for Modification of Luminal Contents of Intestinal Organoids
Organoids are 3D cultures of self-organized adult or pluripotent stem cells with an epithelial membrane enclosing a defined fluid-filled lumen. These organoids have been demonstrated with a wide range of organ...
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Controlling the Potency of T Cell Activation Using an Optically Tunable Chimeric Antigen Receptor
The ability of biological systems to convert inputs from their environment into information to guide future decisions is central to life and a matter of great importance. While we know the components of many o...
