99 Result(s)
within
Protocol
Lu**g Li
Biotechnology
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Protocol
N-Linked Glycan Characterization of Heterologous Proteins
Our laboratory has focused on the re-engineered of the secretory pathway of Pichia pastoris to perform glycosylation reactions that mimic processing of N-glycans in humans and other higher mammals (1,2). A report...
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Phytoremediation of Arsenic-Contaminated Soil in China
Arsenic (As) is a common pollutant of concern in environmental clean up because its contamination is recognized to lead to a variety of cancers, cardiovascular diseases, diabetes, and other health problems. Be...
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Protein Chip for Detection of DNA Mutations
A large number of human genetic diseases, bacterial drug resistances, and single-nucleotide polymorphisms are caused by gene mutations. Rapid and high-throughput mutation detection methods are urgently demande...
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Nanotechnology
Microarrays are important tools for high-throughput analysis of biomolecules. The use of microarrays for parallel screening of nucleic acid and protein profiles has become an industry standard. A few limitatio...
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Mammalian Two-Hybrid Assay for Detecting Protein-Protein Interactions in Vivo
The mammalian two-hybrid system is a very powerful tool to investigate protein-protein interactions in terms of functional domains and identify potential binding ligands and partners of a protein. Compared wit...
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Delivery of DNA into Tumors
Delivery of plasmid DNA encoding therapeutic genes into tumors is one of the main applications of electroporation. This chapter summarizes various investigators' electroporation parameters for intratumoral gen...
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Systemic IL-12 Gene Therapy for Treating Malignancy via Intramuscular Electroporation
Interleukin 12 (IL-12) is effective in treating systemic microscopic malignancies by inducing T helper 1 (TH1) response, inhibiting angiogenesis, and triggering secondary cytokine production. Unfortunately, daily...
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Molecular Beacons for Protein—DNA Interaction Studies
Real-time monitoring of DNA—protein interactions involving molecular beacon (MB) and molecular beacon aptamer (MBA) was discussed in this chapter. MBs are single-stranded oligonucleotide probes with a hairpin ...
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Periodic Assembly of Nanospecies on Repetitive DNA Sequences Generated on Gold Nanoparticles by Rolling Circle Amplification
Periodical assembly of nanospecies is desirable for the construction of nanodevices. We provide a protocol for the preparation of a gold nanoparticle (AuNP)/DNA scaffold on which nanospecies can be assembled i...
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Intratumoral Bleomycin and IL-12 Electrochemogenetherapy for Treating Head and Neck Tumors in Dogs
Bleomycin and Interleukin 12 have been used clinically to treat tumors; however, the co-administration of Bleomycin and Interleukin 12 followed by electroporation has not been tested clinically. In this study,...
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Treatment of SCCVII Tumors with Systemic Chemotherapy and Interleukin-12 Gene Therapy Combination
Cyclophosphamide and Interleukin-12 (IL-12) have been successfully used in clinical trials for treating malignancies. In this study, we explore the coadministration of cyclophosphamide and IL-12 plasmid DNA fo...
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Detection of Enteropathogenic Escherichia coli by Microchip Capillary Electrophoresis
There is always a need to detect the presence of microorganisms, either as contaminants in food and pharmaceutical industries or bioindicators for disease diagnosis. Hence, it is important to develop efficient...
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Cowpea Mosaic Virus-Based Systems for the Expression of Antigens and Antibodies in Plants
This chapter describes the use of Cowpea mosaic virus-based vectors for the production of foreign proteins such as antigens and antibodies in plants. The systems include vectors based on both full-length and d...
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Microchip Capillary Electrophoresis
Microchip capillary electrophoresis (MCE) is gaining popularity due to the developments of simple microfabrication methods under nonstringent laboratory conditions. Moreover, the low material and production co...
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Integrating Waveguide Biosensor
The Integrating Waveguide Biosensor was developed for rapid and sensitive detection of bacterial cells, spores, and toxins. A sandwich format of immunoassay was employed using Salmonella as model. The analyte was...
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Solid-State Nanopore for Detecting Individual Biopolymers
Solid-state nanopores have been fabricated and used to characterize single DNA and protein molecules. Here we describe the details on how these nanopores were fabricated and characterized, the nanopore sensing...
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Multiplex Integrating Waveguide Sensor: Signalyte™-II
A platform to detect multiplex fluorescent labels was developed based on liquid phase implementation of the Integrating Waveguide Sensor detection principles. The liquid sample is held in a capillary cuvette w...
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Biopanning of Phage Displayed Peptide Libraries for the Isolation of Cell-Specific Ligands
One limitation in the development of biosensors for the early detection of disease is the availability of high specificity and affinity ligands for biomarkers that are indicative of a pathogenic process. Withi...
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In Vitro Selection of Protein-Binding DNA Aptamers as Ligands for Biosensing Applications
Aptamers are single-stranded functional nucleic acids that possess cognate ligand recognition capability. These functional nucleic acids have been used for biosensing of a variety of ligands. Aptamers are isol...
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Synthesis of a Virus Electrode for Measurement of Prostate Specific Membrane Antigen
Though relatively unexploited in biosensor applications, phage display technology can provide versatile recognition scaffolds for detection of cancer markers and other analytes. This chapter details protocols...
