17 Result(s)
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Protocol
Correction to: Identification and Localization of Cell Types in the Mouse Olfactory Bulb Using Slide-SeqV2
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Protocol
Medical Image Segmentation Using Deep Learning
Image segmentation plays an essential role in medical image analysis as it provides automated delineation of specific anatomical structures of interest and further enables many downstream tasks such as shape a...
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Correction to: Plant Phosphopeptide Identification and Label-Free Quantification by MaxQuant and Proteome Discoverer Software
In the original version of this book, the title of chapter 13 was published with a typographical error. It has now been rectified in the revised version of this book.
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Protocol
MRI Map** of the Blood Oxygenation Sensitive Parameter T2* in the Kidney: Basic Concept
The role of hypoxia in renal disease and injury has long been suggested but much work still remains, especially as it relates to human translation. Invasive pO2 probes are feasible in animal models but not for hu...
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Development of a Mouse-Adapted MERS Coronavirus
First identified in 2012, Middle East respiratory syndrome coronavirus (MERS-CoV) is a novel virus that can cause acute respiratory distress syndrome (ARDS), multiorgan failure, and death, with a case fatality...
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Evolution of Viral Genomes: Interplay Between Selection, Recombination, and Other Forces
Natural selection is a fundamental force sha** organismal evolution, as it both maintains function and enables adaptation and innovation. Viruses, with their typically short and largely coding genomes, exper...
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Protocol
Correction to: Analysis of Population-Genetic Properties of Copy Number Variations
The original version of the book was inadvertently published with incorrect spelling of the author name “Liu Yang”. The author name has now been corrected.
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Protocol
Correction to: A Practical Guide to Molecular Dynamics Simulations of DNA Origami Systems
Correction to: Chapter 15 in: Giampaolo Zuccheri (ed.), DNA Nanotechnology: Methods and Protocols, Methods in Molecular Biology, vol. 1811, https://doi.org/10.1007/978-1-4939-8582-1_15
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Erratum to: Delivery of the Porcupine Inhibitor WNT974 in Mice
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Studying Coronavirus–Host Protein Interactions
To understand the molecular mechanisms of viral replication and pathogenesis, it is necessary to establish the virus–host protein interaction networks. The yeast two-hybrid system is a powerful proteomic appro...
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Simultaneous Detection and Differentiation of Respiratory Syncytial Virus and Other Respiratory Viral Pathogens
Rapid and accurate detection of respiratory syncytial virus (RSV) provides pathogen-specific diagnosis, allows implementation of appropriate infection control measures, and improves patient management. One dia...
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Identification of Viral Peptide Fragments for Vaccine Development
We report a simple method for identifying foldable viral surface protein fragments in a random but systematic manner. The method involves digestion and reassembly of a target gene to generate a pool of smaller...
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Protocol
Use of GFP to Investigate Expression of Plant-Derived Vaccines
Plants are low-cost bioreactors for the production of various biopharmaceuticals including oral vaccines. Plant-derived oral vaccines are potentially useful in combating viral infections involving mucosal immu...
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Protocol
Mammalian Two-Hybrid Assay for Detecting Protein-Protein Interactions in Vivo
The mammalian two-hybrid system is a very powerful tool to investigate protein-protein interactions in terms of functional domains and identify potential binding ligands and partners of a protein. Compared wit...
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Protocol
A Pancoronavirus RT-PCR Assay for Detection of All Known Coronaviruses
The recent discoveries of novel human coronaviruses, including the coronavirus causing SARS, and the previously unrecognized human coronaviruses HCoV-NL63 and HCoV-HKU1, indicate that the family Coronaviridae har...
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Protocol
Application of siRNA Against SARS in the Rhesus Macaque Model
Containment of the SARS coronavirus (SCV) outbreak was accompanied by the rapid characterization of this new pathogen's genome sequence in 2003, encouraging the development of anti-SCV therapeutics using short...
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Protocol
Sensitive Detection of SARS Coronavirus RNA by a Novel Asymmetric Multiplex Nested RT-PCR Amplification Coupled With Oligonucleotide Microarray Hybridization
We have developed a sensitive method for the detection of specific genes simultaneously. First, DNA was amplified by a novel asymmetric multiplex PCR with universal primer(s). Second, the 6-carboxytetramethylr...
