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  1. No Access

    Protocol

    Construction of Strong Promoters by Assembling Sigma Factor Binding Motifs

    Development of strong promoters is of growing interest in the field of biotechnology and synthetic biology. Here we present a protocol for the construction of strong prokaryotic promoters that can be recognize...

    Yonglin Zhang, Yang Wang, Jianghua Li, Chao Wang, Guocheng Du… in Directed Evolution (2022)

  2. No Access

    Protocol

    Site-Directed Mutagenesis Method Mediated by Cas9

    This study presents an in vitro CRISPR/Cas9-mediated mutagenic (ICM) system that allows rapid construction of designed mutants or site-saturation mutagenesis libraries in a PCR-independent manner. The plasmid ...

    Wan** Chen, Wenwen She, Aitao Li, Chao Zhai, Lixin Ma in Directed Evolution (2022)

  3. No Access

    Protocol

    Production of Genetically Engineered Porcine Embryos by Handmade Cloning

    Genetic engineering is essential to realize the full potentials of pigs both as livestock and as animal models of human disease. With the development of new genetic engineering technologies, such as the cluste...

    Rong Li, **xin Miao, Zhongde Wang in Microinjection (2019)

  4. No Access

    Protocol

    Creating Knockin Alleles in Mouse Embryonic Stem Cells by CRISPR/Cas9-Mediated Homologous Recombination Without Drug Selection

    The rapidly evolving CRISPR/Cas9-mediated genome editing provides the convenience of genome manipulation directly in mouse zygotes for a number of genomic manipulations; but knockins of large insertions prove ...

    **hu Liu, Yan Li, **gqi Lei, Li** Dong in Microinjection (2019)