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Protocol
Digital Microfluidics with an On-Chip Drug Dispenser for Single or Combinational Drug Screening
Rapid and accurate cancer drug screening is of great importance in precision medicine. However, the limited amount of tumor biopsy samples has hindered the application of traditional drug screening methods wit...
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Protocol
Acetylcholinesterase Inhibition Assays for High-Throughput Screening
Acetylcholinesterase (AChE) hydrolyzes acetylcholine (ACh), a vital neurotransmitter that regulates muscle movement and brain function, including memory, attention, and learning. Inhibition of AChE activity ca...
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Protocol
Cell-Based Imaging Assay for Detection of Phospholipidosis
Accumulation of lysosomal phospholipids in cells exposed to cationic amphiphilic drugs is characteristic of drug-induced phospholipidosis. The morphological hallmark of phospholipidosis is the appearance of un...
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Protocol
Cell-Based Assays to Identify Modulators of Nrf2/ARE Pathway
The nuclear factor erythroid 2-related factor (Nrf2) and antioxidant response element (ARE) signaling pathway play an important role in the amelioration of cellular oxidative stress. Thus, assays that detect t...
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Protocol
GFP-LC3 High-Content Assay for Screening Autophagy Modulators
Autophagy plays an important role in maintaining cellular homeostasis. Defects in autophagy have been linked to various human diseases, such as cancer, neurodegenerative diseases, and cardiovascular diseases. ...
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Protocol
DNA-Generated Electric Current Biosensor for Epidermal Growth Factor Receptor 2 (HER2) Analysis
Electrochemical biosensors have been applied in the detection of human serum biomarkers for clinical applications. A kind of signal amplifying method using deoxyribonucleic acid (DNA) as signal probe was devel...
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Protocol
Quantitative and Selective DNA Detection with Portable Personal Glucose Meter Using Loop-Based DNA Competitive Hybridization Strategy
A portable, quantitative, and selective DNA detection biosensor, based on a loop-based DNA competitive hybridization assay and a personal glucose meter (PGM), is an advanced strategy for one-step target DNA re...
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Protocol
Electrochemistry Coupling Localized Surface Plasmon Resonance for Biochemical Detection
Localized surface plasmon resonance (LSPR) associated with metal nanostructures has developed into highly useful sensor techniques. LSPR spectroscopy often shows absorption peaks which could be used for biomed...
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Protocol
Determination of Fraction Unbound and Unbound Partition Coefficient to Estimate Intracellular Free Drug Concentration
Intracellular free drug concentrations are critical to develop pharmacokinetic and pharmacodynamic relationships, estimate therapeutic indices, and predict drug–drug interaction potentials. Free drug concentra...
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Protocol
Contribution of Nontarget Cells to the Disposition, Antitumor Activity, and Antigen-Independent Toxicity of Antibody–Drug Conjugates
After many years of work, antibody–drug conjugates (ADCs) have emerged as an effective strategy for targeting cytotoxic agents to a variety of cancer indications. With nine currently approved ADCs and several ...
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Protocol
Subcellular Drug Depots as Reservoirs for Small-Molecule Drugs
Therapeutic nanoparticles (TNP) have been developed to more efficiently and safely deliver medicines, especially for the treatment of solid cancers. TNP can deliver drugs through extended systemic payload rele...
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Protocol
In Vitro Methodologies to Assess Potential for Transporter-Mediated Drug–Drug Interactions
The importance of membrane transporters and drug-metabolizing enzymes on drug absorption and disposition is widely known. Our current understanding of transporters and enzymes has been achieved largely through...
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Protocol
Expression of IgG Antibodies in Mammalian Cells
Production of IgG antibodies is usually done in mamalian cells. Predominantly, chinese hamster ovary (CHO) cells are used for generating stably transfected cell lines and for manufacturing. In addition to larg...