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Protocol
High-Throughput Screening Assays to Identify Plant Natural Products with Antifungal Properties Against Fusarium oxysporum
Fusarium oxysporum is a cross-kingdom fungal pathogen that not only causes devastating plant vascular diseases but can also opportunistically infect humans. Here we describe two high-throughput screening assays, ...
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Protocol
Metagenomic Assembly: Reconstructing Genomes from Metagenomes
Assembly of metagenomic sequence data into microbial genomes is of critical importance for disentangling community complexity and unraveling the functional capacity of microorganisms. The rapid development of ...
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Protocol
Transcriptome-Wide Detection of Internal N7-Methylguanosine
m7G-seq detects internal 7-methylguanosine (m7G) sites within mRNAs and noncoding RNAs by misincorporation signatures. A chemical-assisted sequencing approach selectively converts internal m7G sites into abasic s...
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Protocol
Simultaneous RNA-DNA FISH
A highly useful tool for studying lncRNAs is , which reveals the localization and quantitative information of RNA and DNA in cellular contexts. However, a simple combination of RNA FISH and DNA FISH often ...
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Protocol
Synthesis and Evaluation of Novel Neamine–Nucleoside Conjugates as Potential Antibiotic Targets for Escherichia coli 16S Ribosomal RNA
Based on the nucleobase rich character of the binding pocket of A-site 16S ribosomal RNA of Escherichia coli, it was proposed that the neamine moiety of synthesized Neamine–nucleoside conjugates could bind to the...
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Protocol
Enantioseparations by Gas Chromatography Using Porous Organic Cages as Stationary Phase
The resolution of chiral compounds into optically pure enantiomers is very important in various fields, such as pharmaceutical, chemical, agricultural, and food industries. Chiral gas chromatography (GC) is on...
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Protocol
A Computational Protocol to Analyze Metatranscriptomic Data Capturing Fungal–Host Interactions
Plant diseases cause significant losses to agricultural production and pose serious threats to food security worldwide. Understanding the mechanism of host–pathogen interaction is essential for the development...
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Protocol
Erratum to: Delivery of the Porcupine Inhibitor WNT974 in Mice
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Protocol
Simultaneous RNA–DNA FISH
A highly useful tool for studying lncRNAs is simultaneous RNA–DNA FISH, which reveals the localization and quantitative information of RNA and DNA in cellular contexts. However, a simple combination of RNA FIS...
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Protocol
Delivery of the Porcupine Inhibitor WNT974 in Mice
We describe here a technique for delivering the porcupine inhibitor WNT974 (formerly LGK974) in mice. The protocol entails once-a-day oral delivery of WNT974 for up to 3 months at a concentration sufficient to...
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Protocol
Development of Antidepressant Drugs Through Targeting α4β2-Nicotinic Acetylcholine Receptors
Nicotinic acetylcholine receptors (nAChRs) represent a family of ligand-gated ion channels that are ubiquitously distributed in the central and peripheral nervous systems. There is a considerable line of evide...
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Protocol
Gene Synthesis Method Based on Overlap Extension PCR and DNAWorks Program
Gene synthesis by chemical methods provides a powerful tool for modifying genes and exploring their structure, expression, and function in the post-genomic era. However, a bottleneck in recent gene synthesis t...
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Protocol
Protein Chip for Detection of DNA Mutations
A large number of human genetic diseases, bacterial drug resistances, and single-nucleotide polymorphisms are caused by gene mutations. Rapid and high-throughput mutation detection methods are urgently demande...